What is the role of caspase-9 in apoptosis?
Active caspase-9 works as an initiating caspase by cleaving, thus activating downstream executioner caspases, initiating apoptosis. Once activated, caspase-9 goes on to cleave caspase-3, -6, and -7, initiating the caspase cascade as they cleave several other cellular targets.
Is caspase-9 cleaved?
Although caspase-9 does not need to be cleaved to be active, apoptotic cell death is always accompanied by autocatalytic cleavage and by further downstream effector caspase-dependent cleavage of caspase-9.
What type of caspase are caspase 8 and 9?
The initiator caspases-8 and -9 normally exist as inactive procaspase monomers that are activated by dimerization and not by cleavage. This process is generalized as an “induced proximity model” (Muzio et al. 1998; Boatright et al. 2003; Chang et al.
What happens when caspases are activated?
Activation of caspases ensures that the cellular components are degraded in a controlled manner, carrying out cell death with minimal effect on surrounding tissues. Caspases have other identified roles in programmed cell death such as pyroptosis and necroptosis.
How is a caspase-9 ( C9 ) antibody produced?
Caspase-9 (C9) Antibody detects endogenous levels of the pro form of caspase-9 as well as cleaved fragments. Monoclonal Antibody is produced by immunizing mice with a recombinant human caspase-9 protein. Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family (1,2).
How to avoid exposure to full length caspase-9?
* Avoid repeated exposure to skin. Caspase-9 Antibody detects endogenous levels of full length caspase-9 (47 kDa) and large fragments of caspase-9. The antibody does not recognize other caspases.
Which is a member of the caspase protease family?
Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family (1,2). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf-1.