What does Neisseria gonorrhoeae look like under a microscope?
Neisseria gonorrhoeae, the gonococcus, is a non-spore-forming, nonmotile bacterium that appears under the microscope as a Gram-negative coccus occurring in pairs (diplococci) with flattening of the adjacent sides. Gonococci are adapted to growth on mucous membranes and cannot tolerate drying.
What shape is Neisseria gonorrhoeae?
Neisseria gonorrhoeae is a Gram-negative coccus, 0.6 to 1.0 µm in diameter, usually seen in pairs with adjacent flattened sides (Figure 1 Left and Fig 2 below). The organism is frequently found intracellularly in polymorphonuclear leukocytes (neutrophils) of the gonorrhea pustular exudate (Figure 1 Right).
What is the colony morphology of Neisseria gonorrhoeae?
Colonial morphology of Neisseria gonorrhoeae: In a humid aerobic environment enriched with 5-10% CO2, colonies are thin, round, translucent, convex or slightly umbonate, with finely granular surface and lobate margins after incubation for 24 hours. The colonies of Neisseria gonorrhoeae are pinkish brown.
Does gonorrhea Gram stain?
The presence of typical gram-negative intracellular diplococci and polymorphonuclear leukocytes on Gram stain from a specimen collected from a symptomatic male establishes a diagnosis of gonorrhea (sensitivity, >95%; specificity, >99%).
Can Gram stain detect gonorrhea?
An endocervical gram stain checks for abnormal bacteria in or around the cervix. Your doctor may also perform this test if they suspect you have an STI. An endocervical gram stain can detect STIs such as: gonorrhea, which is a common STI with painful urination and abnormal discharge.
Is E coli a bacillus?
E coli is a gram-negative bacillus that grows well on commonly used media. It is lactose-fermenting and beta-hemolytic on blood agar. Most E coli strains are nonpigmented.
How do you read Gram stain results?
A Gram stain is colored purple. When the stain combines with bacteria in a sample, the bacteria will either stay purple or turn pink or red. If the bacteria stays purple, they are Gram-positive. If the bacteria turns pink or red, they are Gram-negative.
What does E. coli look like?
E. coli is a rod-shaped bacterium of the Enterobacteriaceae family. It can live in environments with or without air. These bacteria live in the intestines of healthy people and warm-blooded animals.
What color is E. coli when Gram stained?
pink
Gram Staining Coli will appear pink in color. The absence of this (of purple color) is indicative of Gram-positive bacteria and the absence of Gram-negative E. Coli.