What is 1kb DNA ladder?
1 kb DNA Ladder allows for determining the size of double-stranded DNA from 250 – 10,000 base pairs (bp). The ladder consists of 13 double-stranded, blunt-end fragments, of sizes 250, 500, 750, 1000, 1500, 2000, 2500, 3000, 4000, 5000, 6000, 8000, and 10,000 bp (see Figure 1).
How much is 1Kb of DNA ladder?
We recommend loading 10 μl (0.5 μg) of Quick-Load 1 kb DNA Ladder per gel lane. In general, it is recommended to load 1µl per mm of gel lane.
What is a DNA size standard?
A DNA marker (also known as a size standard or a DNA ladder) is loaded into the first well of the gel. The fragments in the marker are of a known length so can be used to help approximate the size of the fragments in the samples.
When to use trackit 1 kb DNA ladder?
TrackIt 1 Kb DNA Ladder is ideal for separation on 0.8–1% agarose gels. The double-stranded DNA ladder can be visualized on 0.8–1% agarose gels after ethidium bromide or SYBR Safe staining. The ladder is designed with a uniform intensity of DNA bands for a clear view of each band.
How many DNA fragments are in a 1 kb DNA ladder?
Invitrogen TrackIt 1 Kb Plus DNA Ladder is ready to use and designed for sizing and approximate quantification of double-stranded DNA in the range of 100 bp to 15,000 bp. 1 Kb Plus DNA Ladder consists of 18 individual chromatography-purified DNA fragments and has a reference band at 1,500 bp for easy orientation.
Is the one KB plus DNA ladder compatible with agarose?
One Kb Plus DNA ladder is compatible with agarose gels cast in lab as well as E-Gel™ pre-cast agarose gels. One Kb Plus DNA ladder is most popular DNA ladder used to estimate molecular mass of double-stranded DNA bands from PCR, restriction digests, and other molecular biology protocols.
Which is the best DNA ladder for PCR?
One Kb Plus DNA ladder is most popular DNA ladder used to estimate molecular mass of double-stranded DNA bands from PCR, restriction digests, and other molecular biology protocols. Unlike some other DNA ladders, 1Kb Plus DNA ladder has been specially designed with bands of round, easy-to-remember sizes.