Where is GAL4 found in the cell?
gene promoter
For study in Drosophila, the GAL4 gene is placed under the control of a native gene promoter, or driver gene, while the UAS controls expression of a target gene. GAL4 is then only expressed in cells where the driver gene is usually active.
What chromosome is GAL4 on?
| Line | Gene | Chr. |
|---|---|---|
| Line | Gene | Chr. |
| CR00323 | Marf | X |
| CR00446 | Dsor1 | X |
What is the function of GAL4?
The broad use of the Gal4 is in yeast two-hybrid screening to screen or to assay protein-protein interactions in eukaryotic cells from yeast to human. In the GAL4/UAS system, the Gal4 protein and Gal4 upstream activating region (UAS) are used to study the gene expression and function in organisms such as the fruit fly.
What is ELAV GAL4?
Likewise, elav-Gal4 lines, which are generally used as postmitotic neuronal driver lines, show expression in neural progenitor cells and nearly all embryonic glial cells. Thus, in the embryo, elav can no longer be considered an exclusive marker or driver for postmitotic neurons.
What does Gal4 bind to?
The GAL4 protein of yeast activates the transcription of several genes involved in galactose metabolism. This event requires that GAL4 bind to upstream activation sites with the consensus sequence 5′-CGGN5(T/A)N5CCG-3′.
How many Gal4 lines are there?
Image data on a representative brain and VNC of each of the 6,650 lines can be viewed at www.janelia.org/gal4-gen1.
How many GAL4 lines are there?
How does enhancer trap work?
Glossary:Enhancer Trap. A type of DNA construct containing a reporter gene sequence downstream of a promoter that is capable of integrating into random chromosomal locations in mouse. Integration of the enhancer trap near an enhancer allows the expression of a new mRNA encoding the reporter gene.
How does Gal4 UAS system work?
The Gal4/UAS system Gal4 is a transcriptional activator that binds to UAS enhancer sequences found in DNA. It then recruits transcription machinery to the site to induce gene expression. You can quickly mate your UAS-YFG line with a variety of Gal4 lines and study gene expression in many different tissue types!
How does Gal4 bind to DNA?
How is the Gal4 protein used in the UAS system?
UAS-GAL4 -Based Gene Expression System. The UAS-GAL4 system is a method of activating gene expression in Drosophila ( Figure 26.2 ). The GAL4 protein, derived from yeast, serves as the transcriptional activator in this system. Its lack of endogenous targets within Drosophila, together with the ability to activate transcription within the fly,
Where are transgenes of interest located in the GAL4 system?
The transgene of interest is placed downstream of a GAL4 upstream activating sequence that supports transcription of the construct only in the presence of GAL4. In this way, the pattern of transgenic protein expression can be tailored, according to the driver line used, to the requirements of the investigator.
Where does the transcription factor GAL4 come from?
The GAL4 protein, derived from yeast, serves as the transcriptional activator in this system. Its lack of endogenous targets within Drosophila, together with the ability to activate transcription within the fly, makes it a favorable tool.
Is there any biological activity of GAL4 in insect cells?
In most cases, GAL4 has no biological activity in insect cells, although in some cases, such as high-level expression in the retina, using GMR-GAL4, may cause mild artifactual phenotypes, possibly due to aggregation of the GAL4 at high concentrations.