Why the nucleic acid gel stain GelRed was used instead of ethidium bromide?
GelRedTM is a highly sensitive fluorescent nucleic acid dye that works when excited by a transilluminator. It was designed to replace EtBr, which is considered to be carcinogenic and mutagenic. GelRedTM is also known to be very sensitive without requiring a destaining step.
What is GelRed why is it used in gel electrophoresis?
General description. GelRed is a sensitive, stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EtBr) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels.
What is nucleic acid stain in gel electrophoresis?
Nucleic acid staining dyes are used for detecting nucleic acids in electrophoresis gels. It was found that GelRed™ was the most sensitive and safest dye to use with UV light excitation, and both GelGreen™ and Diamond™ Nucleic Acid Dye were sensitive and the safer dyes using blue light excitation.
Is GelRed a loading dye?
GelRed® Prestain Plus 6X DNA Loading Dye contains density agents, tracking dyes, and GelRed® dye. The 6X prestain loading dye is added to samples in place of gel loading buffer, and eliminates the need to add fluorescent DNA dye to the agarose gel during casting or after electrophoresis.
What is nucleic acid stain?
Nucleic acid stains enable the researcher to determine sizes of DNA bands produced by standard molecular techniques, such as DNA/RNA extraction, PCR, restriction digestion, and cloning.
Which is the most sensitive nucleic acid gel stain?
GelRed™ Nucleic Acid Gel Stain, 10,000X in Water GelRed from Biotium can be used to stain dsDNA, ssDNA or RNA in agarose gel via either precast or post gel staining. Gel Red is more sensitive than Ethidium bromide.
Can you use GelRed 10, 000x in water?
Biotium also offers GelRed® 10,000X in water (catalog no. 41003) and GelRed® 10,000X in DMSO (catalog no. 41002). GelRed® in water is a newer, safer formulation and our recommended format. We continue to offer GelRed® in DMSO for established users who do not wish to alter their protocols.
What is the patent number for GelRed gel stain?
Storage/Handling: Store at room temperature. Protect from light. GelRed and its uses are covered by US patent numbers 7960498, 7803943, and 8232050. SYBR is trademark of Molecular Probes, Inc.
How is GelRed used to stain agarose gels?
GelRed® is an ultra sensitive, extremely stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EtBr) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. Compatible with downstream gel purification, restriction digest, sequencing, and cloning