What PAS stain detects?
The periodic acid-Schiff (PAS) staining procedure is most commonly used in the histology laboratory to detect glycogen deposits in the liver when glycogen storage disease is suspected. Glycogen granules may also be visible in tumors of the bladder, kidney, ovary, pancreas, and lung.
What is the basis for the PAS stain?
Periodic acid-Schiff reaction (PAS) The Schiff reagent is a bleached basic fuschin that reacts with aldehyde groups. This reaction results in a deep red colour in the section. It is the basis of the PAS stain.
How long does PAS staining take?
Procedure for Periodic Acid-Schiff (PAS) Staining Aldehydration: Place the stain in Schiff reagent for 15 minutes, which turns light pink. Washing: Using lukewarm tap water, wash the stain for 5 minutes, turning it dark pink.
What are the procedures in performing PAS staining?
Procedure:
- Deparaffinize and hydrate to water.
- Oxidize in 0.5% periodic acid solution for 5 minutes.
- Rinse in distilled water.
- Place in Schiff reagent for 15 minutes (Sections become light pink color during this step).
- Wash in lukewarm tap water for 5 minutes (Immediately sections turn dark pink color).
What does PAS stain in kidney?
Renal Pathology. This normal glomerulus is stained with PAS to highlight basement membranes of glomerular capillary loops and tubular epithelium. The capillary loops of this normal glomerulus are well-defined and thin. The endothelial cells are seen in capillary loops.
Does PAS stain nucleus?
Description: This method is used for detection of glycogen in tissues such as liver, cardiac and skeletal muscle on formalin-fixed, paraffin-embedded tissue sections, and may be used for frozen sections as well. The glycogen, mucin, and fungi will be stained purple and the nuclei will be stained blue.
Does PAS stain mucin?
PAS (periodic acid-Schiff): Stains glycogen as well as mucins, but tissue can be pre-digested with diastase to remove glycogen. Stains neutral and acid-simple non-sulfated and acid-complex sulfated mucins.
What does the Diastase step in a PAS stain do why is it useful?
PURPOSE: To determine glycogen by digesting out and staining with PAS stain. PRINCIPLE: The diastase (or a-amylase) act on glycogen to de polymerize it into smaller sugar units, maltose and glucose, that are washed out of the section. One is digested the other is not, both are stained with the PAS stain.
How to get rid of Pas glycogen fungus?
PAS – GLYCOGEN – FUNGUS CONTROL: For staining fungus; use a known positive such as those used for the GMS. Use skin, aorta or normal liver for positive (glycogen) PAS staining. PROCEDURE: 1. Deparaffinize and hydrate to distilled water. 2. Place slides into 0.5% Periodic acid for 5 minutes. 3. Rinse in distilled water. 4.
How is PAS used in the medical field?
PAS stain is mainly used to highlight the molecules (structures) with high percentage of carbohydrate content such as glycogen, glycoproteins, and proteoglycans typically found in connective tissue, glycocalyx and basal laminae. PAS staining can be used to assist in the diagnosis of several medical conditions such as:
When to use PAs for glycogen detection?
PAS (Periodic Acid Schiff) Staining Protocol. NovaUltra Special Stain Kits. Description: This method is used for detection of glycogen in tissues such as liver, cardiac and skeletal muscle on formalin-fixed, paraffin-embedded tissue sections, and may be used for frozen sections as well.
How is the PAS stain used in liver biopsies?
A routine stain for liver and kidney biopsies. PRINCIPLE: The PAS stain is a histochemical reaction in that the periodic acid oxidizes the carbon to carbon bond forming aldehydes which react to the fuchsin-sulfurous acid which form the magenta color. CONTROL: For staining fungus; use a known positive such as those used for the GMS.