What is the role of goat anti rabbit IgG horseradish?
Goat anti-Rabbit IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Summary. Detects rabbit IgG heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with human IgG, mouse IgG, and chicken IgY is observed.
Is Alexa Fluor an antibody?
Alexa Fluor® 488 secondary antibody is ideal immunofluorescence antibodies to get bright and sensitive immunofluorescent staining in cell and tissue fluorescent microscopy.
What is IgG h l?
Anti-IgG (H+L): A secondary antibody with the (H+L) designation targets the heavy and light chains of the IgG molecule (i.e. Fc and Fab regions). These antibodies also react with other classes (e.g. IgE, IgD, etc.) Secondary antibodies specific for the Fc fragment have been adsorbed against F(ab’)2 fragments.
What is Rabbit IgG?
Rabbit IgG is an isotype control used to estimate the non-specific binding of target primary antibodies due to Fc binding or other protein-protein interactions.
What does primary antibody bind to?
antigen
Primary antibodies are immunoglobulins that bind to a specific antigen (protein, peptide, small molecule, etc.) of interest and are typically unconjugated. A good primary antibody recognizes and binds with high affinity and specificity to purify, detect, and measure the antigen.
Are secondary antibodies fluorescent?
Secondary Antibodies are conjugated to a fluorescent dye such as an Alexa Fluor® to generate signal which can be detected using a digital imager. Fluorescent dye-conjugated secondary antibody detects the primary antibody for the protein of interest.
Do mouse and rabbit antibodies cross react?
All Answers (5) Secondary antibodies are usually well developped and show strong binding to the intended primary antibody with minimal background. A secondary anti-rabbit should not bind to either human or mouse antibodies (or hybrids for that matter).