What is the purpose of the tissue cassettes?
Disposable plastic cassettes hold tissue specimens during processing and embedding, as well as in storage. Molded from a special high-density polymer, these patented cassettes keep specimens safely submerged in liquid and are totally resistant to the chemical action of histological solvents.
What is tissue cassette?
Made of acetal polymer Disposable plastic tissue cassettes are suitable for holding and identifying tissue samples in processing, embedding, and sectioning procedures. The cassettes fit securely in microtome chuck adapters. They are molded from a high density polymer that is totally resistant…
What are histology cassettes?
These disposable plastic cassettes hold tissue specimens during processing and embedding, as well as in storage. Molded from a special high-density polymer, they keep specimens safely submerged in liquid and are totally resistant to the chemical action of histological solvents.
What is automatic tissue processing?
Automated tissue processors are of two main types: tissue-transfer machines and fluid-transfer machines. The tissue processor finds applications in histopathology laboratories to automatically prepare tissue samples for laboratory testing, by fixing, dehydrating, clearing, and infiltrating them with paraffin.
What are the dimensions of a tissue cassette?
Histotechnologists retrieve tissues from the specimen jars and transfer the biopsy sample to a standard tissue cassette measuring 3 × 2.5 × 0.4 cm.
How do you label tissue cassettes?
Label cassettes: When labelling cassettes, use only a reagent resistive marker (ex: Statmark Pen) or a #2 hard lead pencil for cassettes, never a pen or Sharpie marker. Solvents used in processing can remove the ink from many “permanent” Sharpie markers.
How many containers are present in automatic tissue processor?
In this type of tissue processor the tissues remain in one container but reagents get changed at scheduled interval. In this process the tissue is changed from one container of reagent to another by hand.
How is embedding done?
Embedding is the process in which the tissues or the specimens are enclosed in a mass of the embedding medium using a mould. Since the tissue blocks are very thin in thickness they need a supporting medium in which the tissue blocks are embedded. This supporting medium is called embedding medium.
What is the maximum size of tissue put into a cassette?
2.5 × 2.0 × 0.4 cm
Tissue slices should not exceed 2.5 × 2.0 × 0.4 cm in a standard tissue processing cassette. Note that appropriately sized tissue should not touch the sides or closed lid of the cassette.
What are advantages of automatic tissue processor?
The device can handle larger number of tissues, process more quickly and produces better quality outcome. Advantages of automated tissue processor – Saves time, decreases human error, effective fluid circulation, Temperature can be adjusted and vacuum/pressure can also be incorporated.
What are disadvantages of automatic tissue processing?
Disadvantages include inability to cut thin sections, storage of blocks in alcohol and speed of technique (which can take several weeks or months). This is harder than paraffin wax and has a lower melting point (46-48oC). Good for cutting hard tissues since it does not crumble.