What is the mordant in acid-fast stain?
During the acid fast stain, heat is used as a mordant to allow the primary stain to penetrate the waxy mycolic acid layer. The heat will prevent the cells from being destained using acid-alcohol. These non-acid fast cells are counterstained with methylene blue.
What is the advantage of the kinyoun staining procedure?
What is the advantage of the Kinyoun staining procedure over the Ziehl-Neelsen method? a complex lipid that is composed of fatty acids and fatty alcohols that have hydrocarbon chains up to 80 carbons in length. the application of heat to cells during staining with carbolfushcin and phenol is not without safety concern.
What is the mordant used in the K technique?
Salts of chromium, aluminum, copper, iron, tin, and cobalt are commonly used as mordants. Since the mordant affects the electron distribution and density within the dye, the color of the dyed fabric tends to change.
What is the mordant used in Gram staining?
Iodine
The mordant is Gram’s Iodine. This binds to the crystal violet making a large complex that adheres to the cell membrane.
What is the primary stain in the kinyoun acid-fast stain?
The primary stain for the Kinyoun procedure is the aniline dye, basic fuchsin, that stains all the cells present red. The unique ability of mycobacteria to resist decolorization by acid-alcohol is why they are termed acid-fast, and will keep their red coloration throughout the staining process.
What is the mordant in capsule staining?
There is no mordant in the capsule staining procedure. A 20% copper sulfate solution serves a dual role as both the decolorizing agent and counterstain. It decolorizes the capsule by washing out the crystal violet, but will not decolorize the cell.
What is Ziehl-Neelsen stain used for?
Ziehl–Neelsen staining is a bacteriological stain used to identify acid-fast organisms, mainly Mycobacteria. It is named for two German doctors who modified the stain: the bacteriologist Franz Ziehl (1859–1926) and the pathologist Friedrich Neelsen (1854–1898).
What is the mordant in the Gram stain?
The mordant is Gram’s Iodine. This binds to the crystal violet making a large complex that adheres to the cell membrane.
What is modified kinyoun?
Modification. The Kinyoun method can be modified as a weak acid fast stain, which uses 5% sulfuric acid instead of hydrochloric acid. The weak acid fast stain in addition to staining mycobacteria will stain organisms that are not able to maintain the carbol fuchsin after decolorizing with HCl.
What is mordant staining?
A mordant is a chemical that fixes or intensifies a dye or stain. In fabric dyeing, a mordant forms a coordination complex with the dye, helping the dye attach to the fabric. Mordants also intensify stains in microbiological slides or tissue preparations.
Where does the name Kinyoun stain come from?
Kinyoun stain is the modified form acid -fast stain and there is no need of heating in this staining procedure so, called cold method. The stain name is from the surname of developer Joseph J. Kinyoun.
How to use Kinyoun acid fast cold stain?
Kinyoun staining Procedure 1 First, prepare the smear on slide. 2 Flood the slide with primary stain, Kinyoun Carbol Fuchsin Stain, and wait for 2 minutes. 3 Rinse the slide with water. 4 Flood the slide with a decolorizer, until no more color drains from the slide (approx 3 to 5 seconds). 5 Again wash the slide with water.
How to stain with Kinyoun carbol fuchsin?
Prepare two smears. Fix with absolute methanol for 1 minute. Flood slide with Kinyoun’s carbol fuchsin, and stain for 5 minutes. Rinse slide briefly (3 to 5 seconds) with 50% ethanol. Rinse thoroughly with water. Decolorize with 1% sulphuric acid for 2 minutes or until no more color runs from the slide.
How is Kinyoun stain used in bone marrow culture?
It can be demonstrated in direct smears by modified Kinyoun stain, in which acid-alcohol decolorizer is substituted with a low-concentration inorganic acid. All bone marrow specimens were positive for MTBC by culture, or were either positive for AFB by Kinyoun stain or contained granulomas.