What is the function of bluing in H&E staining?
One of the steps in the H&E procedure is bluing. As the name implies, this step converts the initial soluble red color of the hematoxylin within the nucleus to an insoluble blue color. The alkaline pH of the bluing solution causes the mordant dye-lake to reform in the tissue and become more permanent.
Why do we blue in Scott’s tap water?
Scott’s solution is of a low alkalinity level. The pH value gets elevated by bluing process, which causes the dye to change from red to blue.
What is a bluing reagent?
Blue reagent is a histological reagent that substitutes tap water and enables fast and accurate bluing of nuclear chromatin and membranes of the cell nucleus. Because of its hardness and alkalinity, tap water changes the color of the nuclei previously stained with hematoxylin.
What does bluing mean?
Definition of bluing : a preparation used in laundering to counteract yellowing of white fabrics.
What will happen if the xylene is not removed before mounting?
Once the slides have been put in the xylene to remove paraffin they should not be allowed to dry out. Particular care must be taken not to let the sections dry at the time of mounting as the xylene easily evaporates and if the section dried before mounting preparation would become useless.
Why is xylene used in staining protocols?
In histology, xylene is used to process and stain tissues. The reason xylene works so well for tissue processing is that it makes tissues transparent so that paraffin can fully envelop the tissue. And when preparing slides for microscopy, xylene can remove any remaining wax from slides.
What is bluing buffer?
Dako Bluing Buffer ensures the proper alkalinity in primary staining resulting in a detailed, crisp and clear nuclear staining. Dako Bluing Buffer is ready-to-use and suitable for use with Dako CoverStainer.
What are the special stains in histopathology?
4.2 Important Histological Stains Used in the Past and Present
- Carmine.
- Hematin and Hematoxylin.
- Silver Nitrate.
- The Hematoxylin and Eosin Procedures.
- Romanowsky Stains–Giemsa Stains.
- Gram Stain.
- Trichrome Stains.
What is the role of grades of alcohol used in staining procedure?
(1) Troubleshooting. Increasing concentrations of alcohol after eosin staining are used to remove water from the tissue section. If all water molecules are not removed from the tissue section, proper clearing cannot be achieved.