What is Invivo gene therapy?
In vivo gene therapy refers to direct delivery of genetic material either intravenously (through an IV) or locally to a specific organ (eg, directly into the eye).
How does in vivo gene therapy work?
In vivo gene therapy means that therapy is administered directly the patient. The targeted cells remain in the body of the patient. With ex vivo gene/cell therapy the targeted cells are removed from the patient and gene therapy is administered to the cells in vitro before they are returned to the patient’s body.
What are the two types of gene theraphy?
There are two different types of gene therapy depending on which types of cells are treated:
- Somatic gene therapy: transfer of a section of DNA to any cell of the body that doesn’t produce sperm or eggs.
- Germline gene therapy: transfer of a section of DNA to cells that produce eggs or sperm.
What are the benefits of in vivo gene therapy?
In vivo gene therapy benefits from its simplicity and the reduced volume of material introduced into the brain (Freeman, 1997). These properties make it an ideal method when the target is a specific, small area of the brain.
What are examples of in vivo gene therapy?
Conversely, in vivo gene therapy in involves the injection of a virus carrying the therapeutic gene directly into a patient’s body. The four most common viral vectors used for both ex vivo and in vivo gene therapy are adenovirus, adeno-associated virus (AAV), lentivirus, and retrovirus.
What is in vivo and ex vivo therapy?
Ex vivo gene therapy involves the genetic modification of cells outside of the body to produce therapeutic factors and their subsequent transplantation back into patients.
What is in vivo experimentation?
When a study is performed in vivo, it can include things like performing experiments in an animal model, or in a clinical trial in the case of humans. In this case, the work is taking place inside a living organism.
Does vivo have electroporation?
In vivo electroporation (EP) is a method that has emerged to facilitate gene delivery of naked plasmid DNA in vivo. Since the first application of the in vivo delivery of chemotherapeutic agents to solid tumor, study of the delivery of plasmid DNA to cancer by EP has grown tremendously.
What is in vitro and ex vivo?
In vitro experiments can be conducted on a wide range of test subjects, from bacteria to cells derived from living organisms. By contrast, ex vivo means “outside of a living body.” In this type of experiment, the living tissues are not created artificially but directly taken from a living organism.
What is in vivo technique?
In vivo refers to when research or work is done with or within an entire, living organism. Examples can include studies in animal models or human clinical trials.
What is in vivo method?
In vivo is Latin for “within the living.” It refers to tests, experiments, and procedures that researchers perform in or on a whole living organism, such as a person, laboratory animal, or plant.