What is Effectene transfection Reagent?
Effectene Transfection Reagent is a nonliposomal lipid reagent for DNA transfection into a broad range of cell types. Due to its low cytotoxicity, Effectene Transfection Reagent is highly suitable for transfection of primary cells and many sensitive cell lines.
How do you transfect HeLa cells?
Transfection of HeLa S3 Cells Plate 4 x 104 cells per well in 0.5 ml of complete growth medium. Cell density should be 50~80% confluent on the day of transfection. For each well of cells to be transfected, dilute 0.25 μg of DNA into 100 μl of Opti-MEM® I Reduced Serum Medium without serum.
How does Effectene work?
The Effectene–DNA complexes are mixed with medium and directly added to the cells. Effectene Reagent spontaneously forms micelle structures that show no size or batch variation, as found with preformulated liposome reagents. This unique feature ensures excellent reproducibility of transfection-complex formation.
Why are HeLa cells used in transfection?
Research with HeLa Cells Being a vicious cancer cell in its own right, the HeLa cell line was originally derived for use in cancer research. Even though HeLa cells are very hardy, the HeLa cell line is fairly easy to transfect and very receptive to viral vectors, making it a popular research tool in virology.
What is a good transfection efficiency?
With cationic lipid-mediated transfection, generally 70–90% confluency for adherent cells or 5 × 105 to 2 × 106 cells/mL for suspension cells at the time of transfection provides good results. Similarly, actively dividing cell lines are more efficiently transduced with viral vectors.
Are HeLa cells hard to transfect?
Transfection difficulty is not limited to immature or primary cells. HeLa, one of biology’s workhorse cell lines, is refractory to high transfection efficiency using chemical methods. Cell lines that have been cultured too long or transfected multiple times might resist transfection as well.
How long does HeLa take to adhere?
some like HeLa only take a couple of hours, others like primary cells often take 12 hours or more.
Can you Trypsinize transfected cells?
All Answers (3) Yes, it works fine to trypsinize and re-plate cells 24 hours after siRNA transfection. I routinely transfected with siRNA in 6-well plates and then trypisinized/pooled and replated cells for analysis at 48 or 72 hours.
What are the features of Effectene transfection reagent?
Effectene Transfection Reagent Feature Specifications Applications Plasmid transfection, protein overexpres Cell type Eukaryotic cells (primary cells and sens Controls Not included Features Non-liposomal lipid formulation, minimal
Can a transfection reagent be used for high throughput screening?
Transfection using Effectene Transfection Reagent requires low amounts of DNA and minimal handling. In addition, removal of transfection complexes is not required, making this reagent highly suitable for high-throughput screening. Effectene Transfection Reagent is available in bulk quantities.
How is transfection used in the medical field?
Transfection is a modern and powerful method used to insert foreign nucleic acids into eukaryotic cells. The ability to modify host cells’ genetic content enables the broad application of this process in studying normal cellular processes, disease molecular mechanism and gene therapeutic effect.
How is transfection used in gene therapy research?
Besides, transfection can be employed as one of the strategies in gene therapy to treat incurable, inherited genetic diseases ( Yao et al., 2008; Yamano, Dai & Moursi, 2010; Tomizawa et al., 2013 ).