What is CART19?
CART19 to Treat B-Cell Leukemia or Lymphoma That Are Resistant or Refractory to Chemotherapy – Full Text View – ClinicalTrials.gov.
Why is CD19 a good target?
CD19 makes an attractive target for cancer therapy because its expression on normal cells is limited to those of B-cell lineage. Furthermore, it is expressed on the vast majority of B-cell malignancies, including 80% of acute lymphoblastic leukemias (ALLs), 88% of B-cell lymphomas, and 100% of B-cell leukemias.
What is T cell malignancy?
A type of cancer that forms in T cells (a type of immune system cell). T-cell lymphomas may be either indolent (slow-growing) or aggressive (fast-growing). Most T-cell lymphomas are non-Hodgkin lymphomas. There are many different types of T-cell non-Hodgkin lymphomas.
Does K562 cells express CD19?
K562-CD19 cell line is derived from K562 which has been engineered to stably express CD19.
What is cytokine release syndrome?
(SY-toh-kine reh-LEES SIN-drome) A condition that may occur after treatment with some types of immunotherapy, such as monoclonal antibodies and CAR-T cells. Cytokine release syndrome is caused by a large, rapid release of cytokines into the blood from immune cells affected by the immunotherapy.
What do CD19 cells do?
CD19 is a biomarker for normal and neoplastic B cells, as well as follicular dendritic cells. CD19 is critically involved in establishing intrinsic B cell signaling thresholds through modulating both B cell receptor-dependent and independent signaling.
Why do car T cells target CD19?
CD19 serves as an ideal target for CAR-directed therapies because it is expressed on most B cell malignancies (including CLL, B-ALL, and many NHL), it is not expressed on hematopoietic stem cells, and elimination of all CD19+ B cells in the body is a manageable on-target treatment effect [25].
Is T-cell lymphoma an autoimmune disease?
Similar to patients with B-cell neoplasms, it appears that patients with T-cell lymphomas also have a relatively high prevalence of autoimmune conditions.
What are Daudi cells?
Daudi is a well characterized B lymphoblast cell line which has been employed extensively in studies of mechanisms of leukemogenesis. The cell line is also a suitable transfection host.
What are signs of cytokine storm?
Cytokine Storm Syndrome Symptoms
- Fevers and chills.
- Fatigue.
- Swelling of extremities.
- Nausea and vomiting.
- Muscle and joint aches.
- Headache.
- Rash.
- Cough.
What kind of cells are cart19 T cells?
These modified cells are called chimeric antigen receptor 19 (CART19) T-cells. At entry subjects will be staged and the suitability of their T cells for CART-19 manufacturing will be determined.
How are cart-19 cells measured in whole blood?
Real Time polymerase chain receptor (RT-PCR) analysis of whole blood will be used to detect and quantify survival of CART-19 TCR:4-1BB and TCR cells over time. For patients with detectable disease, measure anti-tumor response due to CART-19 cell infusions.
Are there any clinical trials for CAR T cells?
Until recently, the use of CAR T-cell therapy has been restricted to small clinical trials, largely in patients with advanced blood cancers.
How are cart-19 T cells purified for use in chemotherapy?
Subjects who have adequate T cells will be leukapheresed to obtain large numbers of peripheral blood mononuclear cells (PBMC) for CART-19 manufacturing. The T cells will be purified from the PBMC, transduced with CART-19 lentiviral vector, expanded in vitro and then frozen for future administration. Chemotherapy will then be given.