What does LC mean in blood test?
During the past decade, liquid chromatography tandem mass spectrometry (LC-MS/MS) has played an increasingly important role in clinical analysis. It is common now to find it in laboratories with applications for endocrinology, toxicology, pharmacology, and therapeutic drug monitoring.
What is the blood test LC-MS MS?
The mass spectrometry (LC-MS/MS) technique provides a more accurate measurement of the low testosterone levels found in women. Depending on the clinical situation, the interpretation of testosterone levels is based on levels of LH, estradiol, SHBG and sometimes free and bioavailable testosterone.
What are LC-MS MS testing for?
LC-MS/MS can be used to verify the amino acid sequence of a protein therapeutic. Typically this involves digesting the sample with a series of peptidases and the fragments can be ran on the LC-MS/MS to verify the expected mass from each fragment.
How does an LC-MS work?
LC/MS/MS utilizes liquid chromatography in a similar manner as described for HPLC. Following the chromatographic separation, molecules are ionized using an energy source. These ionized molecules then pass through a set of magnets, which further separates molecules based on their mass-to-charge ratio.
Is LC-MS an assay?
When combined with stable isotope dilution, LC-MS can be used to develop highly accurate and reproducible assays. Modern mass spectrometers are highly sensitive and LC-MS assays are now viable replacements for many immunoassays.
How long does LC-MS take?
The Availability of Liquid Chromatography LC-MS is highly accurate as well, leading to a reduced likelihood of false positives. Liquid chromatography results can be generated in around 10 to 30 minutes, providing a very fast turnaround.
Where does LC test for?
LC–MS is a bioanalytical method for quantitative analysis of proteins which has several application areas such as biopharmaceutical drug development, drug metabolism and toxicology studies, quantification of drugs in biological fluids (plasma, urine, tissue, etc.), pharmacokinetic studies, bioavailability studies.
How much does a LC-MS cost?
As a rough approximation metals analyses usually run between $25 and $75 per sample, and LC/MS/MS and GC/MS/MS analyses are typically between $100 and $200 per sample. For lipidomics, the cost for running a quantitative analysis (targeted analysis of known lipids) is $120 per sample.
Who uses LC-MS?
LCMS is applied in many industries such as pharmaceuticals, biopharmaceuticals, forensic, industrial, food and environmental sector. For clinical research, the analysis of drugs, vitamins and minerals in whole blood, plasma, serum and urine is conducted routinely using LCMS.
What is LC-MS system?
Liquid Chromatography with tandem mass spectrometry (LC-MS-MS) is a powerful analytical technique that combines the separating power of liquid chromatography with the highly sensitive and selective mass analysis capability of triple quadrupole mass spectrometry.