What are SkBr3 cells?
SkBr3 (also known as SK-BR-3) is a human breast cancer cell line isolated by the Memorial Sloan–Kettering Cancer Center in 1970 that is used in therapeutic research, especially in context of HER2 targeting.
What is cell culture media made of?
Cell culture media generally comprise an appropriate source of energy and compounds which regulate the cell cycle. A typical culture medium is composed of a complement of amino acids, vitamins, inorganic salts, glucose, and serum as a source of growth factors, hormones, and attachment factors.
What are HT 29 cells?
HT-29 is a human colorectal adenocarcinoma cell line with epithelial morphology. These cells are sensitive to the chemotherapeutic drugs 5-fluorouracil and oxaliplatin, which are standard treatment options for colorectal cancer.
What are the main constituents of culture medium?
Technical Support – FAQs
- FORMULATION OF CULTURE MEDIA.
- 1 Nutrients: proteins/peptides/amino-acids.
- 2 Energy: carbohydrates.
- 3 Essential metals and minerals: calcium, magnesium, iron, trace metals: phosphates, sulphates etc.
- 4 Buffering agents: phosphates, acetates etc.
Is MCF-7 primary cell line?
It is ER-positive and progesterone receptor (PR)-positive (8) and belongs to the luminal A molecular subtype (2). MCF-7 is a poorly-aggressive and non-invasive cell line (9), normally being considered to have low metastatic potential (8).
What is Hylecta?
HERCEPTIN HYLECTA (trastuzumab and hyaluronidase-oysk) is approved for the treatment of adults with early-stage breast cancer that is Human Epidermal growth factor Receptor 2-positive (HER2+) and has spread into the lymph nodes, or is HER2-positive and has not spread into the lymph nodes.
What is the SK-BR-3 breast cancer cell line?
SK-BR-3: Human Breast Cancer Cell Line (ATCC HTB-30) 1 Description. SK-BR-3 is a human breast cancer cell line that overexpresses the Her2 (Neu/ErbB-2)… 2 Source. This cell line was established in 1970 from the pleural effusion… 3 Inventors. 4 Key References. Fogh J et al. (1977) One hundred and twenty-seven cultured human tumor cell lines…
What is the effect of Culture Media on transcriptional expression?
Effects of media on transcriptional expression were more pronounced in luminal cell lines (MCF7, SkBr3), than in the basal cell line (MDA-MB-436). Changes in growth media in terms of supplementation and basal medium delayed growth of cells, but improved cell yields.
How does cell culture media affect phenotypic markers?
The formulation of cell growth media has greater effect on the expression of phenotypic markers in luminal, rather than basal cell lines. Media containing mitogens and higher vitamin content improved efficacy of cell culture in terms of cell yields, although greatly increased growth times.
How does culture media affect the growth of breast cancer cells?
Media containing mitogens and higher vitamin content improved efficacy of cell culture in terms of cell yields, although greatly increased growth times. Keywords: breast cancer cell line; culture media; cultured/substance effects; gene expression profiling; tumor cells.