Is DsRed same as RFP?
Red fluorescent protein (RFP) is a fluorophore that fluoresces red-orange when excited. Several variants have been developed using directed mutagenesis. The original was isolated from Discosoma, and named DsRed. Others are now available that fluoresce orange, red, and far-red.
What wavelength is DsRed?
DrFP583 (DsRed) is a bright red fluorescent protein cloned from reef coral (Matz et al. 1999) and has excitation and emission maxima at 558 and 583 nm, respectively.
How many amino acids are in DsRed protein?
All the proteins localized properly, including actin and Golgi fusions, which have not performed well with tetrameric DsRed proteins. The DsRed-Monomer sequence contains 45 amino acid substitutions but maintains a spectral profile virtually identical to other DsRed fluorescent proteins.
What is mCherry sequence?
mCherry is a member of the mFruits family of monomeric red fluorescent proteins (mRFPs). Fluorescent proteins are used to tag components in the cell, so they can be studied using fluorescence spectroscopy and fluorescence microscopy. mCherry absorbs light between 540-590 nm and emits light in the range of 550-650 nm.
Is DsRed a RFP?
Since the discovery of the first red fluorescent protein (RFP), named DsRed, 12 years ago, a wide pallet of red-shifted fluorescent proteins has been cloned and biotechnologically developed into monomeric fluorescent probes for optical microscopy.
Is Cy3 red or green?
Cy3 fluoresces greenish yellow (~550 nm excitation, ~570 nm emission), while Cy5 is fluorescent in the far-red region (~650 excitation, 670 nm emission). Cy3 can be detected by various fluorometers, imagers, and microscopes with standard filters for Tetramethylrhodamine (TRITC).
What is DsRed used for?
DsRed is an interesting marker protein for use in plants because of its red-shifted wavelength spectrum that will avoid damaging cells and tissues by excitation light.
What does the M stand for in mCherry?
sales engineer of Optolong fluorescence… m of mCherry stands for monomer , indicating that the form of mCherry protein is monomer , which is very important in many experimental designs , such as when forming fusion proteins with target maker gene .
How is red fluorescent protein ( dsred2 ) used in plant selection?
Our results suggest that DsRed2 expression provides an early-stage selection tool for the transgenic calli via visual observation. Red fluorescence can be detected not only in callus and somatic embryos but also in most tissues and organs of mature plants.
Where do I enter the Blast Button in protein BLAST?
Enter one or more queries in the top text box and one or more subject sequences in the lower text box. Then use the BLAST button at the bottom of the page to align your sequences.
What is the extinction coefficient of DsRed protein?
Fully matured DsRedin our hands has an extinction coefficient of 75,000 and a fluorescence quantum yield of 0.7, much higher than the values of 22,500 and 0.23 previously reported. We have no explanation for the difference except that the lower values might have been measured on incompletely matured protein.
How to use BLASTp to search protein database?
BlastP simply compares a protein query to a protein database. PSI-BLAST allows the user to build a PSSM (position-specific scoring matrix) using the results of the first BlastP run. PHI-BLAST performs the search but limits alignments to those that match a pattern in the query.