Is crystal violet aqueous?
Crystal Violet, 1% Aqueous, Laboratory Grade, 1 L.
What is the crystal violet solution?
Crystal violet, also known as gentian violet, is a protein dye and is basic in nature. It stains the fatty portions of sebaceous sweat a deep purple color. Crystal violet can also be used to enhance bloody fingerprints.
Is crystal violet soluble in water?
Solubility : Soluble in water (50 mg/ml at 27° C), chloroform, alcohol (partially soluble), and glycerol (partially soluble). Insoluble in ether.
What is the color of crystal violet in basic solution?
At a pH of +1.0, the dye is green with absorption maxima at 420 nm and 620 nm, while in a strongly acidic solution (pH −1.0), the dye is yellow with an absorption maximum at 420 nm….Dye colour.
| Crystal violet (pH indicator) | ||
|---|---|---|
| below pH −1.0 | above pH 2.0 | |
| −1.0 | ⇌ | 2.0 |
How do you dilute crystal violet solution?
For crystal violet solution you dilute 0.125g of crystal violet in 50 ml of 20% methanol. For lysing solution, dilute 5.88 g of sodium citrate in 100ml of D2O, use HCl to adjust pH to 4.2, add 100 ml of 50% ethanol. Concerning plates, the protocol is for 12-well plates, which is indicated in the first step.
How do you use crystal violet solution?
Using a cotton swab to avoid staining your hands, apply gentian violet to the affected area, usually once or twice a day or as directed by your doctor. Apply only to the affected area, and avoid surrounding healthy skin. Wash hands immediately after use unless the area being treated includes the hands.
Is crystal violet a pH indicator?
Crystal violet or methyl violet is used in many applications, including: As a pH indicator (yellow to violet with the transition at a pH = 1.6) In the medical community, it is the active ingredient in Gram’s Stain, used to classify bacteria.
Why crystal violet is harmful for humans?
It acts as a mitotic poison, potent carcinogen and a potent clastogene promoting tumor growth in some species of fish. Thus, CV is regarded as a biohazard substance.
Is crystal violet harmful to humans?
Harmful if inhaled. May be harmful if absorbed through the skin. May cause eye and skin irritation. May cause respiratory tract irritation.
How do you dissolve crystal violet in water?
You can dissolve crystal violet by using a 0.1% SDS solution in water.
Is crystal violet basic or acidic?
Product description: Crystal Violet is used as an acid-base indicator. When dissolved in water, the dye has a blue-violet colour with an absorbance maximum at 590nm and an extinction coefficient of 87,000 M-1 cm-1.
What are the hazards of crystal violet?
Some of those compounds such as crystal violet and malachite green are known for being toxic and carcinogenic, causing health disorders like mutations, cytotoxicity, neurotoxicity, and chromosomal aberrations (Oplatowska et al.
What are the uses for Crystal violet?
Crystal violet or methyl violet is used in many applications, including: As a pH indicator (yellow to violet with the transition at a pH = 1.6) In the medical community, it is the active ingredient in Gram’s Stain, used to classify bacteria The dye destroys cells and is used as a moderate-strength external disinfectant It is widely used as a purple dye for textiles such as cotton and silk
What are the uses for Crystal violet in medical field?
Introduction. Crystal violet was commonly used for the treatment of oral and vaginal candidiasis or for sterilization during operations up to the 1960s.
What is the pH of crystal violet?
It is known in medicine as Gentian violet (or crystal violet or pyoctanin(e)) and is the active ingredient in a Gram stain, used to classify bacteria. It is used as a pH indicator, with a range between 0 and 1.6.