How does nile red staining work?
Nile red is a hydrophobic and metachromatic dye with poor solubility and fluorescence in water, with colour emission varying from deep red to strong yellow gold in hydrophobic environments. Depending on excitation and emission wavelength, the dye has been used to stain different hydrophobic molecules.
What is Nile Red dye?
Nile red (also known as Nile blue oxazone) is a lipophilic stain. Nile red has applications in cell biology, where it can be used as a membrane dye which can be readily visualized using an epifluorescence microscope with excitation and emission wavelengths usually shared with red fluorescent protein.
How do you use Nile Red a selective fluorescent stain for microalgal neutral lipids?
We outline a five-step method to customize the Nile Red protocol to a specific strain: 1) Evaluate the strain’s suitability by checking for the presence of neutral lipid, 2) Select of the best excitation/emission wavelength, 3) Optimization of incubation time, stain carrier, dye concentration, and temperature, 4) …
What ethnicity is Nile red?
He is of German and Japanese descent. His icons are variations of the Belousov–Zhabotinsky reaction.
What is the purpose of Nile red?
Nile red is used to localize and quantitate lipids, particularly neutral lipid droplets within cells.
Is Nile Red water soluble?
What is Nile Red, Product 19123, soluble in? Nile Red is soluble in methanol (1 mg/mL), ethanol (1mg/mL), water (0.2 mg/mL), and 2-methoxyethanol (1mg/mL).
How do you make a red Nile solution?
Prepare a Nile Red solution at a concentration of 10 µg/ml dissolved in alcohol reagent grade ethanol. Store this solution in the dark at 4 °C. Prepare a 30% (v/v) ethanol solution in deionized water and store at 4 °C.
Is Nile red a real chemist?
Nigel Braun began posting his chemistry experiments to YouTube in 2014 under the name NileRed (after the lipophilic stain) with no intention of amassing such a large audience. But now, four years later, he has over 420,000 subscribers that tune in regularly to watch his latest experiment.
What’s the difference between Nile red and Nile blue?
Nile Red has a broad range of emission and can be excited using the 488 nm or 565 nm lasers. Nile Blue is a far-red dye and is excited at 625 nm. Typically, Nile Red fluorescent signal in lipid droplets is observed in the orange-red channels whereas Nile Blue fluorescence is detected in far red channels.
How are Nile red and BODIPY used for lipid analysis?
Their fluorescent staining for lipids offers a rapid and inexpensive analysis tool to measure neutral lipid content, avoiding time-consuming and costly gravimetric analysis. This review collates and presents recent advances in algal lipid staining and focuses on Nile red and BODIPY 505/515 staining characteristics.
How does Nile red affect lipid fluorescence?
Therefore, Nile red does not specifically bind to lipid droplets and these properties may bias the lipid determination with this dye. Additionally, high pigment concentration may also interfere with lipid-induced Nile red fluorescence.
How is Nile red used in medical research?
Later, Nile red was synthesized from Nile blue oxidation and was mainly used as a vital and fluorescent stain for medical and embryological research purposes [ 30 – 32 ].
When did Nile blue lipid stain come out?
In the early nineties, Nile blue was introduced by Smith [ 26] as a histochemical stain for distinguishing between blue-stained neutral lipid and red-stained acid lipid. From then on, it was extensively used in histochemical and physiological studies [ 27 – 29 ].