Which species of bacteria produce the enzyme phenylalanine deaminase?
The genera Proteus, Providencia, and Morganella are related members of the Enterobacteriaceae that are lactose negative, are motile, and produce phenylalanine deaminase. There are several species of Proteus, but Proteus mirabilis and Proteus vulgaris account for the vast majority of clinical Proteus isolates.
Does Staphylococcus contain deaminase enzymes?
Staphylococcus saprophyticus is the only species of Staphylococcus that is typically uropathogenic and possesses a gene coding for a d-serine-deaminase (DsdA).
What is phenylalanine deaminase test used for?
The phenylalanine deaminase (PDA) test is used to differentiate among the urea-positive Gram-negative bacilli based on the ability of the microorganisms to produce phenylpyruvic acid by oxidative deamination.
What color indicates a positive result for the phenylalanine deaminase test?
green colour
Positive test: Production of green colour (Phenylpyruvic acid thus formed reacts with ferric chloride producing a green colored compound thus turning the medium dark green).
Which genera are positive for phenylalanine deaminase quizlet?
What is Phenylalanine? Phenylalanine deaminase is use to differentiate the phenylalanine deaminase positive genera Morganella, Proteus, and Providencia from other members of the family _____________________, which are phenylalanine deaminase negative.
What enzyme does the phenylalanine deaminase test depend on the pathogen to produce?
-To test for phenylalanine deaminase. Some bacteria produce an ENZYME called phenylalanine deaminase which use the amino acid phenylalanine as a SUBSTRATE and catalyzes the removal of an amino group (-NH2) from the phenylalanine. The result products include phenylpyruvic acid and ammonia. You just studied 12 terms!
What is phenylalanine deaminase?
Phenylalanine deaminase (PAD) is an enzyme that removes an amino group from phenylalanine generating phenylpyruvic acid, water, and ammonium ions. This enzyme is used by some bacteria to exploit this amino acid as a carbon and energy source.
What color is a positive pad test?
The test is a firm plastic strip onto which Leukocyte and Nitrite test pads are attached. If the test is positive, the Leukocyte test pad should be beige to dark purple, and the Nitrite test pad should be uniform pink to red.
Which group of tests best identifies the Morganella and Proteus genera?
Cards
| Term Blood culture collection , clean with | Definition 2% iodine and then 70% alcohol |
|---|---|
| Term Which group of test best identifies Morganella and proteus? | Definition Motility, urease and PAD. |
What is the substrate for phenylalanine deaminase?
Phenylalanine agar, also known as phenylalanine deaminase medium, contains nutrients and DL-phenylalanine. The phenylalanine serves as the substrate for enzymes, which are able to deaminate it to form phenylpyruvic acid. Yeast extract in the medium supports the growth of the organisms.
Is morganella H2S positive?
Morganella are motile, non-lactose fermenting gram-negative bacteria, which share with Proteus the capacity for urease production and presence of phenylalanine deaminase. They can be separated from Proteus species by the lack of swarming activity or gelatin liquefaction or H2S production.