Which acid is used in acid decalcification?
The most common acids used for decalcification are 5-10% solutions of hydrochloric acid (HCl), nitric acid, and formic acid. These acids can be used alone or in combinations.
How do you prepare a decalcification solution?
Combine equal parts of the 8% hydrochloric acid solution and the 8% formic acid solution before use. Procedure: Specimens should be decalcified in hydrochloric acid/formic acid working solution 20 times their volume. Change to fresh solution each day until decalcification is complete.
What is the first chemical used in the chemical endpoint decalcification test?
Decalcifying agents – Weak acids Weak acids such as formic acid are popular and are widely used for decalcification. Formic acid can be used as a simple 10% aqueous solution or combined with formalin or with a buffer.
How is decalcification of hydrochloric acid done?
Withdraw approximately 5 ml of the hydrochloric acid/formic acid decalcification solution from under the specimen and place it in a test tube. Add approximately 10 ml of the ammonium hydroxice/ammonium oxalate working solution, mix well and let stand overnight.
What kind of decalcifier is used in pathology?
Strong Acid decalcifiers ( HCl, Hydrochloric acid) are standard in pathology labs. They quickly decalcify but destroy nucleic acids. Moderate Organic Acid decalcifiers ( Formic acid) are slower but also destroy nucleic acids.
Which is the best acid to use for decalcification?
Decalcifying agents – Strong acids Strong acids such as hydrochloric or nitric acid at concentrations up to 10% are the most rapid in action, but if used for an excessive time, will rapidly cause a loss of nuclear staining and can macerate tissues.
When to use ammonium hydroxice for decalcification?
Add approximately 10 ml of the ammonium hydroxice/ammonium oxalate working solution, mix well and let stand overnight. Decalcification is complete when no precipitate is observed on two consecutive days of testing. Repeat this test every two or three days.