What wavelength does GFP emit at?
509 nm
The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The fluorescence quantum yield (QY) of GFP is 0.79.
What is Turbo GFP?
TurboGFP is a bright green fluorescent protein used to study protein function, localization and dynamics in cells. Nanobody based research tools allow reproducible biochemical analysis including mass spectrometry and enzyme activity measurements of TurboGFP fusion proteins.
What is the kDa of GFP?
27 kDa
The green fluorescent protein (GFP), originally discovered in the jellyfish, Aequorea victoria, is composed of 238 amino acids and has a molecular weight of 27 kDa.
What is maxGFP?
maxGFP is a newly discovered green fluorescent. protein (GFP) derived from the copepod Pontellina. sp. maxGFP, just like eGFP, can be used as a posi- tive.
Is kDa the same as g mol?
Measure of molecular weight or molecular mass. One molecular hydrogen molecular atom has molecular mass of 1 Da, so 1 Da = 1 g/mol. Proteins and other molecular macromolecule molecular weights are usually measured in molecular kDa or kD (kilodaltons) – 1000 Da.
How can GFP be denatured?
GFP loses its fluorescence when denatured by temperatures higher than 70 °C,5,6 pH extremes or guanidinium chloride. It recovers its fluorescence partially only when renatured. The thermal stability of isolated and extracted recombinant GFP was evaluated.
How does GFP give green light?
Solutions of purified GFP look yellow under typical room lights, but when taken outdoors in sunlight, they glow with a bright green color. The protein absorbs ultraviolet light from the sunlight, and then emits it as lower-energy green light.
Can GFP and RFP be used together?
GFP and RFP can be used for the generation of fusion proteins without significantly interfering with native protein assembly and function, making them powerful tools for in vivo analyses. While GFP is originated from Aequorea victoria jellyfish, DsRed was isolated from sea anemone Discosoma sp.
What kind of fluorescent protein is turbogfp made of?
TurboGFP is a basic (constitutively fluorescent) green fluorescent protein published in 2006, derived from Pontellina plumata. It is reported to be a rapidly-maturing dimer with moderate acid sensitivity. compare Comparison List Add TurboGFP show comparison clear selection FPbase info
What kind of work can turbogfp be used for?
TurboGFP is a bright green fluorescent protein used to study protein function, localization and dynamics in cells. Nanobody based research tools allow reproducible biochemical analysis including mass spectrometry and enzyme activity measurements of TurboGFP fusion proteins.
What is the sequence similarity between turbogfp and EGFP?
What is the sequence similarity between TurboGFP and EGFP: Sequence comparison Copepod TurboGFP is evolutionarily distant from jellyfish-derived fluorescent proteins such as EGFP: Turbo-GFP shares only ~20 % sequence identity with the commonly used GFP variants.
What does green fluorescent protein ( GFP ) stand for?
Green Fluorescent Protein (GFP) is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo.