What is the size of FITC?
Fluorescein isothiocyanate (FITC)–dextrans can be obtained in different molecular sizes (molecular weights ranging from 3 kDa to 2,000 kDa) and have the additional advantage that, depending on their molecular size, they can be used to test both solute and ion permeability (low molecular weight dextrans) and protein …
What does FITC stand for?
FITC
| Acronym | Definition |
|---|---|
| FITC | Future Innovation Technology Creativity |
| FITC | Fishery Industrial Technology Center (University of Alaska Fairbanks) |
| FITC | Feed Ingredient Trading Corporation (Delmar, NY) |
| FITC | Foreign Investor Tax Credit (New Zealand) |
How do you use FITC?
Dissolve the FITC in anhydrous DMSO at 1 mg/ml. Note: This should be prepared fresh for each labeling reaction. 3. For each 1 ml of protein solution, add 50 µl of FITC solution, very slowly in 5 µl aliquots while gently and continuously stirring the protein solution.
What wavelength is FITC?
FITC emits fluorescence from 475 to 650 nm, peaking at 525 nm, which falls in the green spectrum.
What is the molecular weight of FITC?
389.382 g/mol
Fluorescein isothiocyanate/Molar mass
Is FITC hydrophobic?
FITC (fluorescein isothiocyanate) conjugated with dextran has a molecular weight of 4000–40,000 Da and, for sodium fluorescein, the molecular weight is around 376 Da (Wilhelm et al., 2011). Both of these compounds show fluorescence and are hydrophilic in nature; the excitation wavelength is between 490 and 520 nm.
Is FITC the same as fluorescein?
Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. FITC has excitation and emission spectrum peak wavelengths of approximately 495 nm and 519 nm, giving it a green color.
What is FITC A in flow cytometry?
Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. FITC has excitation and emission spectrum peak wavelengths of approximately 495 nm and 519 nm, giving it a green color. Like most fluorochromes, it is prone to photobleaching.
What is FITC filter?
It is optimised for fluorescein isothiocyanate (FITC) and other fluorophores such as rsGFP, BODIPY, 5-5-FAM, Fluo-4 and Alexa Fluor 490. The set contains excitation, emission and dichroic filters designed to fit in standard filter cubes from the leading microscope and third-party manufacturers.
Is Alexa Fluor 488 same as FITC?
Molecular Probes™ Alexa Fluor® 488 dye—with nearly identical spectral properties and quantum yield as fluorescein (FITC)—produces brighter, more photostable conjugates that are ideal for imaging and other applications requiring increased sensitivity and environmentally insensitive fluorescence detection.
What is the excitation and emission range of FITC?
FITC is a fluorescent reagent for the labeling of amines. Excitation maximum ~ 495 nm; emission maximum ~ 525 nm. Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
How is FITC spectrum subtracted from PE signal?
Shown in red is the portion of the FITC spectrum that will be detected in the PE detector (585/40) and that must be subtracted from the PE signal using compensation. This process becomes even more complicated when photons from multiple dyes are detected in each PMT.
What kind of photons does FITC emit?
For example, FITC emits photons that are green, yellow and orange, all of which can be detected on a multidetector instrument with the corresponding detectors (Figure 10). In some experiments FITC may be combined with other dyes, for example PE, that emit yellow and orange photons.