What is the nature of the ion exchanger in DEAE?
DEAE-C is a weak anion exchanger. This exchange is utilized to separate proteins that have faintly differing charges. Like all anion exchangers, the resin carries a positive charge that interacts favorably with negative charges. The positive charge of DEAE cellulose is due to a protonated amine group.
What is the binding capacity of DEAE Sephacel?
10 mg
SPECIFICATIONS
| Flow Rate | <50 cm/h |
|---|---|
| pH Range | 2 – 12 |
| Total Ionic Capacity | 0.10–0.14 mmol Cl-/mL |
| Binding Capacity | 10 mg Thyroglobulin (MW 669 000)/mL drained medium, 160 mg HSA (MW 68 000)/mL drained medium |
| Storage Temperature | 4 – 8 °C |
What is resin in ion exchange method?
An ion-exchange resin or ion-exchange polymer is a resin or polymer that acts as a medium for ion exchange. The beads are typically porous, providing a large surface area on and inside them the trapping of ions occurs along with the accompanying release of other ions, and thus the process is called ion exchange.
How do you make Deae resin?
Suspend the resin in 2 CV of 0.1 M NaOH containing 0.5 M NaCl for 10 min. 1 and pour the slurry into a Buchner funnel (volume = 3 CV) while applying GENTLE suction, and allowing a flow of 1 V buffer in 5 minutes. Continue pouring in slurry until all the resin is added to the funnel.
What is CM resin?
Description. Macro-Prep CM Resin is a weak cation exchange resin that meets the demands of analytical, semipreparative, and process-scale applications. This rigid, macroporous hydrophilic support provides exceptional capacity, resolution, and throughput in a chemically and mechanically stable form.
Which is a strong anion exchange column, DEAE or Sepharose?
Diethylaminoethyl-sepharose (DEAE-sepharose) is a strong anion exchange column, where DEAE covalently binds to sepharose. Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
How is DEAE-C used in ion exchange chromatography?
Schematic structure of DEAE-C: positively charged diethylaminoethanol groups can bind negative ions. Diethylaminoethyl cellulose (DEAE-C) is a positively charged resin used in ion-exchange chromatography, a type of column chromatography, for the separation and purification of proteins and nucleic acids.
Which is positively charged resin is used in ion exchange chromatography?
Diethylaminoethyl cellulose (DEAE-C) is a positively charged resin used in ion-exchange chromatography, a type of column chromatography, for the separation and purification of proteins and nucleic acids.
How is HITRAP DEAE Sepharose FF used in chromatography?
HiTrap DEAE FF is prepacked with DEAE Sepharose Fast Flow, and is a weak anion exchanger for small-scale protein purifications as well as screening of binding and elutions conditions. Convenient and affordable for fast, easy ion exchange separations either alone or connected in series. The industry standard for ion exchange chromatography.