What is SNP probe?
SNP microarrays Probes are designed to have the SNP site in several different locations as well as containing mismatches to the SNP allele. By comparing the differential amount of hybridization of the target DNA to each of these redundant probes, it is possible to determine specific homozygous and heterozygous alleles.
How long are TaqMan probes good for?
Custom TaqMan primers and probes are good for at least 12 months from the date of receipt if stored properly.
What is SNP array genotyping?
In molecular biology, SNP array is a type of DNA microarray which is used to detect polymorphisms within a population. Around 335 million SNPs have been identified in the human genome, 15 million of which are present at frequencies of 1% or higher across different populations worldwide.
How does the TaqMan SNP genotyping assay work?
The TaqMan® SNP genotyping technology utilizes the 5’ nuclease activity of Taq polymerase to generate a fluorescent signal during PCR. For each SNP, the assay uses two TaqMan® probes that differ in sequence only at the SNP site, with one probe complementary to the wild-type allele and the other to the variant allele.
What kind of assay is used for SNP genotyping?
Taq DNA polymerase’s 5’-nuclease activity is used in the Taqman assay for SNP genotyping. The Taqman assay is performed concurrently with a PCR reaction and the results can be read in real-time as the PCR reaction proceeds.
What kind of DNA is used for TaqMan bound allele discrimination?
The Molecular Genomics Core (MGC) offers Taqman-bound allele discrimination analysis as a service. Samples should be submitted in the form of genomic DNA either frozen or as an ethanol precipitate.
Which is the best genotyping assay for drug metabolism?
TaqMan®Drug Metabolism Genotyping Assays (DME Genotyping Assays) offer a comprehensive collection of inventoried assays that are optimized for genotyping SNPs, insertions and deletions (indels), and multi‑nucleotide polymorphisms (MNPs) in drug metabolism‑related genes.