What is serial dilution PDF?
It is a method of diluting a stock solution where concentration decreases by the same quantity in each successive step. Materials required: stock solution, test tubes, pipettes, beaker, and distilled water.
What is serial dilution method?
Serial dilution is a common technique used in many immunologic procedures. A small amount of serum or solute can be serially diluted by transferring aliquots to diluent. These dilutions can be done in microtiter plates or test tubes depending on the volumes of sample and diluent used.
How do you calculate serial dilution?
In serial dilutions, you multiply the dilution factors for each step. The dilution factor or the dilution is the initial volume divided by the final volume. For example, if you add a 1 mL sample to 9 mL of diluent to get 10 mL of solution, DF=ViVf = 1mL10mL=110 .
How do you calculate the serial dilution?
Dilution calculations can be performed using the formula M 1 V 1 = M 2 V 2. A serial dilution is a series of stepwise dilutions, where the dilution factor is held constant at each step.
How do you calculate serial dilutions?
A serial dilution is any dilution in which the concentration decreases by the same factor in each successive step. In serial dilutions, you multiply the dilution factors for each step. The dilution factor or the dilution is the initial volume divided by the final volume. #DF = V_i/V_f# = #(1″mL”)/(10″mL”) = 1/10#. This is a 1:10 dilution.
What is the purpose of serial dilution?
Serial dilutions are used to accurately create extremely diluted solutions, as well as solutions for experiments that require a concentration curve with an exponential or logarithmic scale. Serial dilutions are widely used in experimental sciences, including biochemistry, pharmacology, microbiology, and physics.
What is a serial dilution test?
A serial dilution test can measure the concentration of a target microbe in a sample of water or food. Several tubes are inoculated each with prespecified amounts of the initial sample.