What is recombinant Taq polymerase?
Thermo Scientific Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. Recombinant Taq DNA Polymerase is the ideal tool for standard PCR of templates 5 kb or shorter.
What is Taq polymerase used for in PCR?
“The function of Taq DNA polymerase in PCR is to amplify or synthesize DNA or gene of interest for various downstream applications. It’s a type of thermostable DNA polymerase, work at a higher temperature as well.”
Why do you use Taq DNA polymerase for PCR reactions?
The DNA polymerase typically used in PCR is called Taq polymerase, after the heat-tolerant bacterium from which it was isolated (Thermus aquaticus). This heat-stability makes Taq polymerase ideal for PCR. As we’ll see, high temperature is used repeatedly in PCR to denature the template DNA, or separate its strands.
What is Taq DNA polymerase and why is it so special?
Taq DNA polymerase is the most common enzyme used for PCR amplification. This enzyme is extremely heat resistant with a half-life of 40 minutes at 95°C. This will result in the amplification of non-specific targets that can be overcome by the use of a ‘hot-start’ PCR technique (Mullis 1991).
What does Taq polymerase stand for?
Taq DNA Polymerase is a highly thermostable recombinant DNA polymerase. It is named after Thermus aquaticus, the heat-tolerant bacterium from which it isolates itself.
Is Taq polymerase DNA dependent?
Taq DNA polymerase, one of the first thermostable DNA polymerases to be discovered, has been typecast as a DNA-dependent DNA polymerase commonly employed for PCR.
How does Taq DNA polymerase work?
Once primers are attached, the Taq polymerase takes its position on the strand to produce the new strands by adding the dNTPs. This leads to the production of new complementary DNA (cDNA) strands. The newly synthesized strands thus act as templates in the next cycle of PCR. After each cycle, the DNA doubles.
What is the purpose of Taq polymerase in a PCR reaction chegg?
Taq polymerase is a synthetic enzyme that produces DNA strands at a faster rate than natural polymerases. b. Taq polymerase is a heat-stable form of DNA polymerase that can function after exposure to the high temperatures necessary for PCR.
Why is Taq polymerase important?
Due to its key role in synthesizing and amplifying new strands of DNA, Taq DNA Polymerase is essential to Polymerase Chain Reaction (PCR). Like other DNA polymerases, Taq Polymerase can only produce DNA if it has a primer, a short sequence of 20 nucleotides that provide a starting point for DNA synthesis.
What are the differences between Taq and Pfu polymerases?
The main difference between Pfu and alternative enzymes is that Pfu has superior thermostability and proof-reading properties. Unlike Taq DNA polymerase, Pfu DNA polymerase also possesses 3′->5′ exonuclease proofreading activity, resulting in PCR fragments with fewer errors than Taq-generated PCR inserts.