What is IC50 value for antioxidant activity?
IC50 is the concentration required to result in a 50% antioxidant activity, for example, a 50% DPPH free radical scavenging, or 50% reducing power, compared with control. A smaller IC50 means higher antioxidant activity.
How do you calculate IC50 for antioxidants?
you can calculate the IC50 as follows: IC50 = (0.5 – b)/a. You replace (y) by 50 in the equation y = 1.3534 x + 0.58 and you deduce ( x) .
What is the correlation between TPC and antioxidant activity?
These results show that TPC significantly correlated with antioxidant activities (R2 > 0.7820). However, flavonoids contributed not much to the antioxidant activities; hence the correlation coefficients were relatively low (R2 > 0.3540).
What is IC50 of ascorbic acid?
The IC50 value of ascorbic acid was less than 10 approx to be 6.1 μg/mL. IC50 value was observed 28 μg/mL for the MeOH extract and 36 for BuOH extract. From Figure 1 and Table 1, it is observed that both extracts show significant DPPH radical scavenging property.
What is a good IC50 value DPPH?
According to28, extracts which possess IC50 values ranging from 50 to 100 mg / mL is considered to exhibit intermediate antioxidant activity. Meanwhile, extracts with IC50 value ranging between 10 to 50 mg / mL is considered to possess strong antioxidant acitivity (Table 3).
What is a good IC50 value?
3.2 Determination of EC50/IC50/CC 50
| IC 50 or CC50 | Criteria | |
|---|---|---|
| Isolated compound | Extract | |
| < 1 μM | – | Excellent or potent activity |
| 1–20 μM | < 10 μg/mL | Good activity or very strong cytotoxicity |
| 20–100 μM | 10–50 μg/mL | Moderate activity |
Is DPPH an antioxidant?
DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) free radical method is an antioxidant assay based on electron-transfer that produces a violet solution in ethanol (10). This free radical, stable at room temperature, is reduced in the presence of an antioxidant molecule, giving rise to colorless ethanol solution.
How is antioxidant concentration measured?
ORAC Assay (The Oxygen Radical Absorbance Capacity) ORAC assay is a method for quantifying the antioxidant strength of substances. It involves combining the sample to be tested (i.e. the antioxidant) with a fluorescent compound as well as a compound that generates free radicals at a known rate.
How is TPC value calculated?
x = y/14.26. Total volume of your extract was 40 ml, so now you need to calculate the quantity of total polyphenols in it which equals 40x. You used 2 g of dried leaf so if you want to calculate total polyphenols in 1 g you need to: 40x/2 g = 20 x (mg of total polyphenols expressed as catechine)/1 g sample.
How is IC50 value calculated for anticancer activity?
IC50 values were calculated by linear approximation regression of the percentage survival versus the drug concentration. Cell survival rate (%)=(a-c)/(b-c) x100 (a=absorbance at each concentration of the anticancer reagent, b=absorbance at 0 µM of the anticancer reagent, and c=absorbance of the blank).
Should IC50 be low or high?
In most cases, the IC50 of the best candidate compound should be lower to 10 micromolar, acceptable for NIH to screen the NCI60 program. In addition, the drug design should think over the related problem about the absorption, distribution, metabolism and excretion in our body.