What is Cytofluorometric analysis?
Cytofluorometry is a convenient technique to study cell death and apoptosis on a per-cell basis. In the simplest method, a chromatinophilic dye such as PI is used to label cells and apoptotic cells with diminished PI fluorescence and forward light scatter are compared to cells in the main peak (G1).
What is a flow cytometer used for?
Flow Cytometry is a technique used to detect and measure physical and chemical characteristics of a population of cells or particles. In this process, a sample containing cells or particles is suspended in a fluid and injected into the flow cytometer instrument.
What is fluorescent flow cytometry?
Fluorescence flow cytometry (FFC) is used to analyse physiological and chemical properties of cells. It can also be used to analyse other biological particles in urinalysis analysers. It provides: Information about cell size and structure. Information about a cell’s interior.
What is cellular granularity?
Side scatter measurement provides information about the internal complexity (i.e. granularity) of a cell. The interface between the laser and intracellular structures causes the light to refract or reflect. Cellular components that increase side scatter include granules and the nucleus (1).
What is cytometry Wikipedia?
Cytometry is the measurement of the characteristics of cells. Cytometry is used to characterize and count blood cells in common blood tests such as the complete blood count.
How does a flow cytometer work?
Using a flow cytometer machine, cells or other particles suspended in a liquid stream are passed through a laser light beam in single file fashion, and interaction with the light is measured by an electronic detection apparatus as light scatter and fluorescence intensity.
What is SSC and FSC?
The light scatter is measured by two optical detectors. One detector measures scatter along the path of the laser (1). This parameter is referred to as forward scatter (FSC). The other detector measures scatter at a ninety degree angle relative to the laser (1). This parameter is called side scatter (SSC).