What do Heinz bodies indicate?
Heinz bodies are indicative of oxidative injury to the erythrocyte. They are clumps of irreversibly denatured hemoglobin attached to the erythrocyte cell membrane.
What do Howell-Jolly bodies indicate?
A Howell–Jolly body is a cytopathological finding of basophilic nuclear remnants (clusters of DNA) in circulating erythrocytes. During maturation in the bone marrow, late erythroblasts normally expel their nuclei; but, in some cases, a small portion of DNA remains.
What do pappenheimer bodies indicate?
Pappenheimer bodies are seen in certain types of anemia that are characterized by an increase in the storage of iron, such as sideroblastic anemia and thalassemia. These inclusions are also seen in the peripheral blood following a splenectomy.
When are Howell-Jolly bodies seen?
This peripheral blood smear shows 2 RBCs that contain Howell-Jolly bodies (arrowheads). Howell-Jolly bodies are remnants of RBC nuclei that are normally removed by the spleen. Thus, they are seen in patients who have undergone splenectomy (as in this case) or who have functional asplenia (eg, from sickle cell disease).
Which anemia would test positive for Heinz bodies?
The presence of Heinz bodies represents damage to hemoglobin and is classically observed in G6PD deficiency, a genetic disorder that causes hemolytic anemia.
Can you see Heinz bodies on Wright stain?
Heinz bodies are erythrocyte structures composed of precipitated denatured hemoglobin. On Wright’s-stained blood smears, they appear as a rounded structure protruding from the margin of an erythrocyte or as a small somewhat refractile spot within the cell.
What stain is used to see Howell-Jolly bodies?
Howell–Jolly bodies are nuclear remnants. They are small, round cytoplasmic inclusions that stain purple on a Romanowsky stain.
What kind of stain does Heinz body have?
Heinz bodies stain the same red-pink color of mature RBCs with routine Wright stains, and stain pale basophilic with Diff-Quik stain. With Diff-Quik stain, Heinz bodies may be seen both as round projections from the RBC membrane and as clear round inclusions that have not yet projected out from the RBC membrane.
Can a Heinz body be seen in Wright’s stained smears?
Heinz bodies may be seen in Wright’s stained smears, especially if they are large and distort the red cell membrane. In contrast, small “endogenous” Heinz bodies, such as those that can be seen in cats, are harder to visualize.
What does a Heinz body look like on a RBC?
Heinz bodies appear as singular, round projections from the RBC membrane. They may be quite small or very large and prominent. In some cases, a single RBC may have several small Heinz bodies. Heinz bodies stain the same red-pink color of mature RBCs with routine Wright stains, and stain pale basophilic with Diff-Quik stain.
How can you tell if a Heinz body is a human?
Heinz bodies are most easily identified on a wet-mount blood film, prepared by mixing one drop of new methylene blue stain with one drop of blood on a glass slide, topped with a cover slip. Heinz bodies are erythrocyte structures composed of precipitated denatured hemoglobin.