Why SH-SY5Y cells should be differentiated?
It is therefore critical that when designing an experiment focused on testing neurons in vitro, SH-SY5Y cells should be differentiated in order to obtain the most accurate results for translation and comparison to in vivo models.
Are SH-SY5Y cells neuronal?
SH-SY5Y human neuroblastoma cells are commonly used as neuronal models. Here, we examined different aspects of SH-SY5Y cell differentiation. Various differentiation protocols have been proposed previously, including treatments with retinoic acid, brain-derived neurotrophic factor (BDNF), cholesterol and oestradiol.
What are IMR 32 cells?
A human neuroblastoma cell line (IMR-32), when differentiated, mimics large projections of the human cerebral cortex and under certain tissue culture conditions, forms intracellular fibrillary material, commonly observed in brains of patients affected with Alzheimer’s disease.
Are neuroblasts progenitor cells?
In invertebrates such as Drosophila, neuroblasts are neural progenitor cells which divide asymmetrically to produce a neuroblast, and a daughter cell of varying potency depending on the type of neuroblast. Vertebrate neuroblasts differentiate from radial glial cells and are committed to becoming neurons.
What is the function of neuroblasts?
Neuroblasts feature prominently in the explosion of growth that takes place while embryos develop. But they also hang around into adulthood, helping the production of new brain cells and aiding recovery from brain injury and stroke.
How many dopaminergic neurons are there?
Representation of dopaminergic pathway system in human brain. Considerable differences exist in the numbers of midbrain dopaminergic cell bodies in various mammals ranging from about 45,000 in the rat, 165,000 in the macaca monkey, to 590,000 in human beings (German & Manaye, 1993).
What should be considered when using SH-SY5Y cells?
Three important characteristics of SH-SY5Ycells should be considered when using these cells in in vitro studies. First, cultures include both adherent and floating cells, both types of which are viable.
How long does it take SH-SY5Y cells to grow?
The secret is in the media, the SH-SY5Y cells will grow very rapidly in Opti-MEM made by Invitrogen. The drawback is that it is much more expensive than the regular MEM or F12 or whatever other media that is recommended by ATCC. The OPTI-MEM contains a proprietary blend and your cells will definitely be ready for sub-culture within a week.
What happens to the proliferation rate of sh-sy5ycells?
Differentiation of SH-SY5Ycells also induces a decrease in proliferation rate, as cells are withdrawn from the cell cycle, and an increase in the activity of neuron specific enolase (NSE), the dominant enolase-isoenzyme present in neuronal and neuroendocrine tissues [2, 6].
How does SH-SY5Y differentiate to a cholinergic neuron?
SH-SY5Y cells differentiate primarily to a cholinergic neuron phenotype in response to RA treatment, as evidenced by increased expression of choline acetyl transferase (ChAT) activity and vesicular monamine transporter (VMAT) expression [7, 15].