What is the selective media for Bacillus subtilis?
Bacillus Differentiation Agar is recommended for differentiation between Bacillus cereus and Bacillus subtilis based on mannitol fermentation. Yeast autolysate provide necessary nitrogenous source for growth of Bacillus. Magnesium sulphate and Potassium chloride supports sporulation.
What Agar does Bacillus subtilis grow on?
The strain of Bacillus subtilis found on MacConkey agar and Chapman agar medium did not grow; however, it grow better on TSA agar medium containing 5% fetal calf serum with circular ridges, smooth, moist, sticky and medium-sized colonies.
What is the selective media for Bacillus species?
Polymyxin pyruvate egg-yolk mannitol–bromothymol blue agar (PEMBA) is a diagnostic selective medium that has been formulated for the enumeration of Bacillus cereus in foods.
How do you identify Bacillus species?
Bacillus species are rod-shaped, endospore-forming aerobic or facultatively anaerobic, Gram-positive bacteria; in some species cultures may turn Gram-negative with age. The many species of the genus exhibit a wide range of physiologic abilities that allow them to live in every natural environment.
What are the characteristics of Bacillus?
What is the colony morphology of bacillus?
Bacillus anthracis — Colony Characteristics Colonies are nonhemolytic, flat or slightly convex with irregular edges and ground-glass appearance. There are often comma shaped projections from the colony edge producing a “Medusa-head” colony. Colonies have a sticky consistency when manipulated with a loop.
What is Bacillus cereus selective agar?
Bacillus cereus Selective Agar is based on the highly specific diagnostic and selective PEMBA medium, developed by Holbrook and Anderson1 for the isolation and enumeration of Bacillus cereus in foods. These features distinguish Bacillus cereus from other Bacillus spp. except Bacillus thuringiensis.
What is the selective medium for Bacillus subtilis?
I think you can use the MYP agar, it´s a selective medium to Bacillus cereus but also allow the development of Bacillus subtilis. Smt. K. W. College Sangli 416416.
What is the morphology of Bacillus subtilis germ?
Bacillus subtilis is a typical germ, which is rod-shaped and Gram-positive. When cultured on ordinary nutrient agar, the morphology circular colony of this bacteria is rough, opaque, fuzzy white or slightly yellow with jagged edges [1, 7].
How is differential medium used in microbiology lab?
This is a differential medium. It tests an organism’s ability to ferment the sugar glucose as well as its ability to convert the end product of glycolysis, pyruvic acid into gaseous byproducts. This is a test commonly used when trying to identify Gram-negative enteric bacteria, all of which are glucose fermenters but only some of which produce gas.
Which is the differential ingredient in MacConkey agar?
MacConkey agar. The selective ingredients are the bile salts and the dye, crystal violet which inhibit the growth of Gram-positive bacteria. The differential ingredient is lactose. Fermentation of this sugar results in an acidic pH and causes the pH indicator, neutral red, to turn a bright pinky-red color.