What is the Gal4 UAS system used for?
The GAL4-UAS system is a biochemical method used to study gene expression and function in organisms such as the fruit fly. It has also been adapted to study receptor chemical-binding functions in vitro in cell culture.
Why are Drosophila used in genetic experiments?
Genetic manipulation melanogaster only has four pairs of chromosomes compared to 23 pairs in humans. This simplicity was one of the reasons why they were first used in genetic studies; Drosophila genes could be mapped easily to investigate genetic transmission.
What UAS genetics?
An upstream activating sequence or upstream activation sequence (UAS) is a cis-acting regulatory sequence. It is distinct from the promoter and increases the expression of a neighbouring gene.
Why are fruit flies ideal for genetic research?
Fruit flies have a very simple genetic structure, which makes them ideal for genetic research. It is useful to study mutant fruit flies, as their quick reproduction rate allows scientists to observe the advantages and disadvantages of certain mutations.
What did Thomas Morgan prove?
He showed that genes are linked in a series on chromosomes and are responsible for identifiable, hereditary traits. Morgan’s work played a key role in establishing the field of genetics. He received the Nobel Prize for Physiology or Medicine in 1933.
Where does the transgene come from?
A transgene is a gene or a segment of DNA that is introduced into the genome of an organism. It comes from an organism especially of another species. This gene is introduced to another organism that makes the latter acquire new property or capability.
Is UAS a transgene?
For example, the GAL4/UAS system to direct transgene expression in a temporally and spatially controlled manner allows expression of toxic gene products, and modeling of neural features of disease (Brand and Perrimon, 1993).