What is T7 RNA polymerase used for?
T7 RNA Polymerase is used for in vitro mRNA synthesis and is highly specific for the T7 phage promoter. Applications include: Radiolabeled RNA probe preparation. RNA generation for studies of RNA structure, processing and catalysis.
What is an RNA antibody?
Anti-RNA antibodies can target several distinct antigens and proteins, such as ribonucleic acid and RNA-associated proteins. RNA-associated protein targets include RNA polymerase II (POLR2A), RNA dependent polymerases, and RNA-binding proteins.
Where is T7 RNA polymerase found?
The RNA polymerase gene of bacteriophage T7 has been cloned into the plasmid pBR322 under the inducible control of the lambda PL promoter. After induction, T7 RNA polymerase constitutes 20% of the soluble protein of Escherichia coli, a 200-fold increase over levels found in T7-infected cells.
What is RNA polymerase III antibody?
Anti-RNA polymerase III antibodies (ARA) are a specific marker for Systemic Sclerosis (SSc), associated to severe disease with major organ and diffuse cutaneous involvement.
What is mRNA used to treat?
Over the past decade, mRNA — short for messenger ribonucleic acid — has emerged as a promising next-generation technology for creating a new class of medications to treat cystic fibrosis, heart disease, rare genetic conditions, and even some cancers.
How is T7 virus transmitted?
Bacteriophage T7 (or the T7 phage) is a bacteriophage, a virus that infects bacteria. It infects most strains of Escherichia coli and relies on these hosts to propagate. Bacteriophage T7 has a lytic life cycle, meaning that it destroys the cell it infects….T7 phage.
| Escherichia virus T7 | |
|---|---|
| Species: | Escherichia virus T7 |
Does T7 virus have a vaccine?
T7 phage may be potentially useful as a delivery vector for DNA vaccine transfer. The surface display capability of T7 phage also enlarge the use in vaccine design, for it can surface display antigen epitope and carry DNA vaccine within one particles.
What does T7 infect?
Bacteriophage T7 (or the T7 phage) is a bacteriophage, a virus that infects bacteria. It infects most strains of Escherichia coli and relies on these hosts to propagate.
Which is a full length T7 RNA polymerase antibody?
Recombinant T7 RNA Polymerase (full-length). (A)is a phase contrast photo showing a viral plaque (vaccinia virus-T7) that has formed on a monolayer of BHK-21 cells. On the right is a photo of the same plaque labeled using the T7RNAP antibody provided by CABT-B8990.
What kind of antibody is used for t7rnap?
On the right is a photo of the same plaque labeled using the T7RNAP antibody provided by CABT-B8990. The vaccinia virus has been engineered to express T7RNAP, thus infected cells are labeled red.
What are the risks of anti RNA polymerase III antibodies?
(1) Numerous studies have shown that systemic sclerosis patients with anti-RNA polymerase III have an increased risk of the diffuse cutaneous form of scleroderma, with a high likelihood of skin involvement and hypertensive renal disease. (1,4) A positive result supports a possible diagnosis of systemic sclerosis (see Cautions).
Which is a specific subunit of RNA polymerase III?
RNA polymerase III is a complex, 16-subunit enzyme directing transcription of small, stable nontranslated RNA genes: tRNA, 5S rRNA, Alu RNA, U6 snRNA, and 7SK snRNA genes. The immunodominant epitope for autoantibodies with anti-RNA polymerase I/III specificity has been identified on the RNA polymerase-specific subunit RPC155.