What is pETDuet?
pETDuet™-1 is designed for the coexpression of two target genes. The vector also carries the pBR322-derived ColE1 replicon, lacI gene and ampicillin resistance gene. This vector can be used in combination with pACYCDuet™-1 (Cat. No. 71147-3) in an appropriate host strain for the coexpression of up to 4 target genes.
What is a duet vector?
The Duet vectors are T7 promoter expression vectors, each designed to coexpress two target proteins in E. coli. The vector encodes two multiple cloning sites (MCS), each of which is preceded by a T7 promoter, lac operator and ribosome binding site (rbs).
What is a pET vector?
pET Bacterial Recombinant Protein Vector. Overview. The pET vector system is a powerful and widely used system for expressing recombinant proteins in E. coli. The gene of interest is cloned into the pET vector under the control of the strong bacteriophage T7 transcription and translation regulatory system.
What is difference between plasmid and vector?
Main Difference – Plasmid vs Vector The main difference between plasmid and vectors is that plasmid is an extra-chromosomal element of mainly bacterial cells whereas vector is a vehicle that carries foreign DNA molecules into another cell. Plasmids can also be used as vectors.
What is a pET plasmid?
Noun. pET plasmid (plural pET plasmids) Any of a family of vectors for protein expression where the expression of the insert DNA is controlled by the T7 promoter, a phage promoter.
How does IPTG induce protein expression?
IPTG or Isopropyl β-D-1-thiogalactopyranoside is a chemical reagent mimicking allolactose, which removes a repressor from the lac operon to induce gene expression. It acts as an inducer to initiate the transcription of genes in the lac operon.
What is IPTG in biotechnology?
IPTG (Isopropyl ß-D-1-thiogalactopyranoside), is a molecular biology reagent. This compound is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon and it is therefore used to induce protein expression where the gene is under the control of the lac operator.
What is the difference between SnapGene and SnapGene viewer?
SnapGene is molecular biology software that allows users to plan, visualize, and document molecular biology procedures. SnapGene Viewer is software that allows you to create, browse, and share richly annotated DNA sequence files.
What is a vector and plasmid?
Plasmid and vector are two types of self-replicative DNA molecules. Plasmids are the extra-chromosomal elements, naturally occurring inside the bacterial cells. Vectors are artificially-introduced DNA molecules into the cells. Plasmids do not carry essential genes for the functioning of the bacterial cells.
Is the petduet-1 vector available on Addgene?
This vector is NOT available from Addgene. pETDuet-1 is designed for the coexpression of two target genes. pETDuet-1 carries the pBR322-derived ColE1 replicon, lacI gene and ampicillin resistance gene.
What are the target genes for petduet-1 plasmid?
Plasmid: pETDuet-1. pETDuet-1 is designed for the coexpression of two target genes. pETDuet-1 carries the pBR322-derived ColE1 replicon, lacI gene and ampicillin resistance gene.
How are the duet vectors used in E coli?
The Duet vectors are T7 promoter expression vectors, each designed to coexpress two target proteins in E. coli. The Duet vectors carry compatible replicons and antibiotic resistance markers and may be used together in appropriate host strains to coexpress up to eight proteins.