What is oligonucleotide probe?
Oligonucleotide probes are short stretches of single-stranded DNA or RNA used to detect the presence of complementary nucleic acid sequences (target sequences) by hybridization. Oligonucleotide probes are usually labelled, for example with radioisotopes, epitopes, biotin or fluorophores to enable their detection.
What is an in situ probe?
= In situ hybridization is a laboratory technique in which a single-stranded DNA or RNA sequence called a probe is allowed to form complementary base pairs with DNA or RNA present in a tissue or chromosome sample. The probe has a chemical or radioactive label attached to it so that its binding can be observed.
What are the different types of in situ hybridization probes?
In situ hybridization probes
- Double-stranded DNA (dsDNA) probes.
- Single-stranded DNA (ssDNA) probes.
- RNA probes (riboprobes)
- Synthetic oligonucleotides (PNA, LNA)
What is oligonucleotide sequencing?
Oligonucleotides, or oligos, are short single strands of synthetic DNA or RNA that serve as the starting point for many molecular biology and synthetic biology applications! From genetic testing to forensic research and next-generation sequencing, an oligo may very well be the starting point.
What are RNA probes?
RNA probes are stretches of single-stranded RNA used to detect the presence of complementary nucleic acid sequences (target sequences) by hybridization. RNA probes are usually labelled, for example with radioisotopes, epitopes, biotin or fluorophores to enable their detection.
What are probes in PCR?
Probes are fluorescently labelled DNA oligonucleotides. They are designed to bind downstream of one of the primers during the PCR reaction and to give a fluorescent signal during the reaction. The 5′ end of the probe is labelled with a fluorescent reporter molecule.
What is mRNA in situ hybridization?
In situ hybridization is a powerful technique for identifying specific mRNA species within individual cells in tissue sections, providing insights into physiological processes and disease pathogenesis. This method avoids freezing tissue and thus associated freeze artifacts.
What are polynucleotides in biology?
A polynucleotide molecule is a biopolymer composed of 13 or more nucleotide monomers covalently bonded in a chain. DNA (deoxyribonucleic acid) and RNA (ribonucleic acid) are examples of polynucleotides with distinct biological function.
How are oligonucleotides formed?
Oligonucleotide synthesis is the chemical synthesis of relatively short fragments of nucleic acids with defined chemical structure (sequence). Products are often isolated by high-performance liquid chromatography (HPLC) to obtain the desired oligonucleotides in high purity.
What kind of probes are used in in situ hybridization?
In situ hybridization probes. Double-stranded DNA (dsDNA) probes. Single-stranded DNA (ssDNA) probes. RNA probes (riboprobes) Synthetic oligonucleotides (PNA, LNA)
Which is the best labeling kit for oligonucleotides?
Alexa Fluor Oligonucleotide Amine Labeling Kits, which use familiar chemical labeling of amine-terminated oligonucleotides to prepare the best singly labeled fluorescent oligonucleotide conjugates.
What is the purpose of RNA in situ hybridization?
RNA ISH (RNA in situ hybridization) is used to measure and localize RNAs (mRNAs, lncRNAs, and miRNAs) within tissue sections, cells, whole mounts, and circulating tumor cells (CTCs). In situ hybridization was invented by Mary-Lou Pardue and Joseph G. Gall.
Are there molecular probes that can label nucleic acids?
To facilitate the preparation of optimally labeled nucleic acids, we offer several modified nucleotides, as well as a wide selection of nucleic acid labeling kits that contain reactive versions of Molecular Probes fluorophores. Our available Invitrogen Molecular Probes technologies include: