What is lupus erythematosus cell test?
The lupus erythematosus (LE) cell test (synonyms: LE prep, LE phenomenon; CPT No. 85544, LE Cell Prep) is a diagnostic test for systemic lupus erythematosus (SLE) that is based on an in vitro immunologic reaction between the patient’s autoantibodies to nuclear antigens and damaged nuclei in the testing medium.
How is LE cell formed?
In any event, two separate serum factors arise in SLE which react with DNA. One reacts with DNA alone and possesses little if any ability to induce LE cell formation. The other reacts with the DNA and the histone of nucleoprotein and is respon sible for formation of LE cells.
Why LE cell test is done?
Lupus erythematosus (LE) cell testing was once performed to diagnose systemic lupus erythematous but has been replaced for this purpose by antinuclear antibody (ANA) testing. Negative findings on LE cell testing exclude a diagnosis of systemic lupus erythematosus (SLE). The presence of LE cells indicates lupus.
What antibody gives rise to LE cell?
Antinuclear antibody cause damage to the nucleus which degenerates and gives rise to a homogeneous body called a hematoxylin body. Mature neutrophilic polymorphonuclear leukocyte, which has phagocytized a spherical, homogeneous-appearing inclusion (hematoxylin body).
What does ANA test?
An ANA test detects antinuclear antibodies (ANA) in your blood. Your immune system normally makes antibodies to help you fight infection. In contrast, antinuclear antibodies often attack your body’s own tissues — specifically targeting each cell’s nucleus.
What is LE cell phenomenon?
The LE cell phenomenon was based on the observation that cellular components released during cell death (particularly nuclei) can be phagocytosed by neutrophils in the mileu of certain plasma factors.
Where are Le cells found?
LE cells have since been found in synovial fluid, cerebrospinal fluid and pericardial and pleural effusions from patients with SLE.
What is Le Cell describe in details?
An LE cell (Lupus Erythematosus cell), also known as Hargraves cell, is a neutrophil or macrophage that has phagocytized (engulfed) the denatured nuclear material of another cell. The denatured material is an absorbed hematoxylin body (also called an LE body).