What is Capto resin?
Capto chromatography resins give you increased productivity compared with Sepharose resins, through high binding capacity and high flow rates. The high chemical stability of Capto resins lets you perform cleaning-in-place procedures with ease.
How does Capto adhere work?
Capto adhere is a multimodal anion exchanger. In multimodal chromatography, the ligand interacts with the target molecule through multiple types of interactions. Ionic interactions are commonly involved, but other interactions such as hydrogen bonding and hydrophobic interactions can be significant.
What is Capto adhere?
Capto adhere is a multimodal strong anion exchanger for BioProcess applications. It was originally designed for post-protein A purification of MAbs at process scale in flowthrough mode. However, Capto adhere can also be used in bind/elute mode and for applications other than MAbs.
What is chromatography resin?
Chromatography resins are media used to capture and polish mAbs, antibody fragments, vaccines, and other biomolecules using a stationary phase. There are several resin types for both analytical and purification purposes.
What is chromatography resin made of?
There are multiple types of ion-exchange resin. Most commercial resins are made of polystyrene sulfonate.
What resins are used in chromatography?
Size exclusion or gel filtration chromatography resins include Bio-Gel® P Polyacrylamide Gels for separating high molecular weight molecules and Bio-Beads S-X Resin for the separation of lipophilic ploymers. Macro-Prep HIC Resins are used for protein, polypeptide, and nucleic acid purification.
What is resin in ion exchange chromatography?
An ion-exchange resin or ion-exchange polymer is a resin or polymer that acts as a medium for ion exchange. It is an insoluble matrix (or support structure) normally in the form of small (0.25–1.43 mm radius) microbeads, usually white or yellowish, fabricated from an organic polymer substrate.
What binds to an anion exchange column?
Anion-exchange chromatography is a process that separates substances based on their charges using an ion-exchange resin containing positively charged groups, such as diethyl-aminoethyl groups (DEAE). Anion exchange resins will bind to negatively charged molecules, displacing the counter-ion.
What is the action of cation-exchange resin?
A cation-exchange resin is a cross-linked polymer with negatively charged structural units. The resin can exchange bound Na+ (Kayexalate) or Ca2+ (calcium resonium) for cations including K+. The purpose of using resins is to enhance the elimination of K+ from the gastrointestinal tract.
Do cations have more protons than electrons?
A cation has more protons than electrons, consequently giving it a net positive charge.