What is BV421?
BD Horizon™ Brilliant Violet™ 421 (BV421) (Exmax 407 nm/Emmax 421 nm) is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences. This dye offers improved brightness over Pacific Orange™ and reduced spillover into the FITC channel when compared to AmCyan.
What are the types of fluorochrome?
In general, fluorochromes can be divided into 5 broad categories, which are discussed below.
- Fluorescent Proteins. Fluorescent proteins can be categorized into two groups.
- Synthetic Small Molecules.
- Quantum Dots.
- Polymer Dyes.
- Tandem Dyes.
What is used as a fluorochrome?
Fluorescent dyes (or fluorochromes) are commonly used as detection reagents in various applications such as cellular imaging and flow cytometry. Fluorochromes absorb light energy of a specific wavelength and re-emit it at a longer wavelength. This fluorescence is the read-out signal provided by the instrument.
Is BV421 a tandem dye?
(Ex max 407 nm/Em max 786 nm) is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em Max at 786 nm. Across the past 40 years flow cytometry solutions have kept pace with the accelerating speed of discovery in life sciences.
Is BV421 fixable?
However, BV421™ is consistently demonstrated to be compatible with viability probes Fixable Live/Dead Blue or DAPI excited off the UV laser. The BV421 channel (450/50 off the violet laser) can appear to have higher background than is a result of much higher autofluorescent emission in that range.
Is BV421 Pacific Blue?
BV421™ has an emission spectrum that is more narrow than that of Pacific Blue™, thus, there is less spillover into neighboring channels, such as AmCyan or Horizon™ V500.
Is FITC a fluorochrome?
FITC (Ex-Max 494 nm/Em-Max 520 nm): Fluorescein isothiocyanate – has a very high efficiency of energy transfer from absorbed to emitted light and is one of the most commonly used fluorochromes.
What kind of filter does BD BV421 use?
Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector.
What’s the excitation maximum of the BV421 dye?
This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter).
What to look for in a fluorochrome panel?
Cytek Aurora Fluorochrome Selection Guidelines Fluorochrome Signatures Dyes can be used in combination as long as they have a distinct spectrum signature. In designing a multicolor panel, you should look for dyes with unique spectra and consider their impacts on other dyes (see slide 18).
What kind of antibody is brilliant violet 421?
Brilliant Violet™ 421 is a trademark of Sirigen. The 2.4G2 antibody specifically recognizes a common nonpolymorphic epitope on the extracellular domains of the mouse FcγIII (CD16) and FcγII (CD32) Receptors.