What is a Millipore filter used for?
The Millipore Filter technique is a new method for the concentration, removal, and enumeration of bacteria in liquids and air. Its application to the evaluation of the sanitary condition of pipe lines cleaned in place is disaussed, together with other possible applications to water, milk, and food bacteriology.
How do you wet a hydrophobic filter?
Hydrophobic membranes are wettable with alcohols. If the polymer is compatible with alcohols, it can be wet first with the alcohol and then equilibrated in water prior to filtering the aqueous fluid. For many applications, this is impractical; the membrane must be directly wettable with the aqueous fluid.
What are syringe filters used for?
Syringe filters are single-use, membrane-based devices used for the removal of particulate impurities from liquid and gas samples prior to analysis by methods such as HPLC, ion chromatography, gas chromatography, ICP, and dissolution testing.
How does Millipore filter work?
Trademark name of a filter usually composed of cellulose acetate with controlled pore size that separates particles above specific sizes from the solutions that flow through.
What is the pore size of Millipore filter?
A: Millipore membrane filters (diameter, 25 mm; pore size, 0.45 µm) point-inoculated with bacteria (ca.
Can bacteria pass through 0.45 filter?
Bacteria growing on MF-Millipore filters (thickness, 150 micro m) passed through the underlying membrane by their infiltration activity. Bacillus subtilis, Staphylococcus aureus, Klebsiella pneumoniae, and Escherichia coli passed through a 0.45- micro m pore size filter within 48-96 h.
What is the difference between hydrophobic and hydrophilic filters?
Hydrophilic filters are easily wet with water. Hydrophobic filters will not wet in water but will wet in low surface tension liquids, for example, organic solvents such as alcohols. Once a hydrophobic filter has been wetted, aqueous solutions also will pass through.
What are hydrophobic filters used for?
Hydrophobic filters are commonly used in gas vent functions within medical devices. These filters allow you to vent out air or other gases while retaining the water based fluids that will be used to aid in the treatment of patients.
How do I choose a syringe filter?
Syringe filter size is determined by the size of your sample. In other words, the amount of volume you plan to push through the filter. As a general rule, use a syringe filter with a greater membrane area if you plan to push a high volume of aqueous solution through it.
How to sterilize a MF Millipore membrane filter?
Glue the filter to one side of a diffusion chamber ring without hole (catalog number PR00 014 00), using MF cement (catalog number XX70 000 00), and let dry. Sterilize this assembly, along with another membrane filter of the same type, by exposure to ethylene oxide gas or ultraviolet light.
What are the autoclavable conditions for millipore filters?
Millipore’s MF, Durapore, Fluoropore and Mitex filters are all autoclavable. Standard conditions of 121-126C for 20-30 min generally work quite well. However, the filters should be autoclaved on a “slow exhaust (liquid) cycle.
How big are the grids on a Millipore filter?
The grids are 3.08mm x 3.08mm square. A grid line is 44um in width and there is are average of 169 gridded squares per filter. Please note however that the actual filtration area and therefore the total number of squares in that area is dependent on the filter holder.