What does enzyme specificity mean?
Specificity is a property of the enzyme and describes how restrictive the enzyme is in its choice of substrate; a completely specific enzyme would have only one substrate. Consequently, a single serine protease may act on various substrates although at different rates.
What does the Km value mean?
Km value is numerically equal to the substrate concentration at which the half of the enzyme molecules are associated with substrate. km value is an index of the affinity of enzyme for its particular substrate.
What are specific enzymes?
Each different type of enzyme will usually act on only one substrate to catalyse one biological reaction. Enzymes are specific because different enzymes have differently shaped active sites. The shape of the active site of an enzyme is complementary to the shape of its specific substrate .
What is enzyme KM value?
The rate of reaction when the enzyme is saturated with substrate is the maximum rate of reaction, Vmax. This is usually expressed as the Km (Michaelis constant) of the enzyme, an inverse measure of affinity. For practical purposes, Km is the concentration of substrate which permits the enzyme to achieve half Vmax.
What is meant by KM value in enzyme reaction?
Km value is equal to the substrate concentration at which half of the enzyme active sites are saturated with the substrate. It tells about the affinity of enzymes for their substrate. Km is the concentration of substrate at which half of the Vmax is attained.
What are the 4 types of enzyme specificity?
There are 4 types of specificity – absolute, group, linkage, and stereochemical. Not all enzymes work on all substrates.
What is specificity in science?
Specificity of a test is the proportion of who truly do not have the condition who test negative for the condition. Mathematically, this can also be written as: A positive result in a test with high specificity is useful for ruling in disease.
What does KM mean in enzyme kinetics?
Michaelis constant
This is usually expressed as the Km (Michaelis constant) of the enzyme, an inverse measure of affinity. For practical purposes, Km is the concentration of substrate which permits the enzyme to achieve half Vmax.
What does KM and Vmax mean?
Vmax is the maximum rate of an enzyme catalysed reaction i.e. when the enzyme is saturated by the substrate. Km is measure of how easily the enzyme can be saturated by the substrate. Km and Vmax are constant for a given temperature and pH and are used to characterise enzymes.