What are HL-60 cells used for?
The HL-60 cell line is a human leukemia cell line that has been used for laboratory research on blood cell formation and physiology. HL-60 proliferates continuously in suspension culture in nutrient and antibiotic chemicals. The doubling time is about 36–48 hours.
Where do HL-60 cells come from?
HL60 is a human cell line derived from peripheral blood lymphocytes of a 36 year old woman suffering from acute promyleocytic leukaemia. It was one of the first human myeloid leukaemia cells to be established as a continuous suspension cell culture which had previously been difficult to achieve.
What are HL 60 cells and why do they make a good model system for stem cell research?
The HL-60 cell line has proven useful in the study of cell formation and differentiation of normal and of course leukemic cells. This is because HL-60 cells can be differentiated through the myeloid pathway to cells such as neutrophils quite easily using DMSO, DMF and other inducers such as retinoic acid.
Why are HL-60 cells good for stem cell research?
The HL60 cell genome contains an amplified c-myc proto-oncogene; c-myc mRNA levels are correspondingly high in undifferentiated cells but decline rapidly following induction of differentiation. These features have made the HL60 cell line an attractive model for studies of human myeloid cell differentiation.
How do I confirm my APL diagnosis?
The diagnosis of APL is based on an evaluation of the clinical presentation, morphology, immunophenotyping, karyotype, RT-PCR, FISH and immunofluorescence with anti-PML monoclonal antibodies. The redundancy of diagnostic tests is beneficial because of the broad and indistinct characterization of the disease.
When should you suspect APL?
Thus, in standard-risk APL, hydroxyurea should be initiated if the WBC count rises to >10 × 109/L, and in high-risk disease, anthracycline chemotherapy during induction should be considered.
What kind of cell line is HL60?
HL60 (human promyelocytic leukemia cell line) HL60 is a human caucasian promyelocytic leukemia cell line (Figure 7.3(a)), derived from a 36-year-old woman with acute PML at the National Cancer Institute. HL60 cells are predominantly a neutrophilic promyelocyte, used for laboratory research on how certain kinds of blood cells are formed.
What kind of culture is used for HL60?
HL60 proliferates continuously in suspension culture in a RPMI medium supplemented with fetal bovine serum, L-glutamine, HEPES and antibiotics [ 36 ]. A hemocytometer was used to obtain a final concentration of 1 × 10 6 cells/ml.
How to grow HL60 cells in priming media?
1. Radiolabel RBL-2H3 or HL60 cells by addition of 1 μCi/mL [ 3 H]Inositol to growth medium and culture for 48 h and then resuspend and incubate in priming media for 90 min at 37 °C. 2.
How are HL-60 cells differentiated in serum-free cells?
HL-60 cells were cultured in serum (FBS) or serum-free (X-VIVO) medium and differentiated using all-trans retinoic acid (ATRA) or dimethyl sulfoxide (DMSO). dHL-60 cells were stimulated with phorbol 12-myristate 13-acetate (PMA) or Ca2+ionophore (CI). Cell differentiation and apoptosis]