What are Cos 7 cells used for?
The COS-7 (CV-1 in Origin with SV40 genes) cells are known as non-steroidogenic cells because they are derived from kidney cells and the kidney is defined as a non-steroidogenic organ. Therefore, COS-7 cells are used for transfection experiments to analyze the actions of functional molecules including steroids.
What is a transfection experiment?
Transfection is a common laboratory cell culture technique used in many research areas, drug discovery, and development. In vitro transfection refers to the delivery of cargo molecules (such as nucleic acids – DNA or RNA) into cultured cells (usually cancer cell lines).
What is a cell transfection?
Broadly defined, transfection is the process of artificially introducing nucleic acids (DNA or RNA) into cells, utilizing means other than viral infection.
Why are hek293 cells used for transfection?
Firstly, they are very easy to grow and to maintain, with high reproducibility, which makes them preferable over other less-robust and slow-growing cell lines. Furthermore, they are very efficient at protein production and accessible for transfection.
Do cells grow after transfection?
It is quite normal to observe growth inhibition or even decreased cell viability post-transfection. Expression of your plasmid interferes with the proteostasis of your cells. HEK 293 also typically become less adherent after transfection.
How can I increase my transfection?
Improving the Success of Your Transfection
- Transfect healthy, actively dividing cells at a consistent cell density.
- Transfect using high-quality DNA.
- Optimize the amount of DNA used to transfect cells.
- Optimize the transfection reagent:DNA ratio.
- Optimize cell number per well when transfected.
Why are COS-7 cells used for transfection experiments?
The combination of fibroblastic-like growth and virus susceptibility make COS-7 a great choice for transfection experiments for DNA plasmids and mutations to the SV40 virus. The SV40 virus continues to be utilized due to its putative involvement in human cancers.
What kind of cell line is COS-7?
COS-7 is one of several types of COS ( C V-1 in O rigin with S V40 genes) cell lines commonly used today.
What does Cos stand for in cell culture?
Thus, COS is an acronym for C V-1 in O rigin, carrying S V40. In culture, COS-7 cells characteristically display adherent growth to glass and plastic surfaces and are fibroblast-like.
How is a controlled addition used in transfection?
The controlled addition generates a precipitate that is dispersed onto the cultured cells. The precipitate is taken up by cells via endocytosis or phagocytosis. Calcium phosphate transfection is routinely used for both transient and stable transfection of a variety of cell types.