How to elute HA-tagged protein?
Use 4 X 1 column volumes of 1 mg/ml HA peptide solution to elute HA-tagged proteins. Elution yields will increase by incubating the peptide on the column for 10-15 minutes at 30-37ºC for each elution fraction. Reconstitute to 1 mg/ml using TBS or distilled water.
How do you purify the flag tagged protein?
How to Purify FLAG-tagged Proteins? FLAG-tagged recombinant protein can be affinity purified directly from a cell culture lysate or supernatant. The FLAG-tagged protein binds to the FLAG-tag specific monoclonal antibody conjugated on an agarose gel.
Is ha a peptide?
The HA peptide, NH2-YPYDVPDYA-COOH, is a synthetic peptide used to competitively elute fusion proteins containing the influenza hemagglutinin (HA) tag bound to immobilized anti-HA affinity resins.
Does flag tag affect protein activity?
FLAG epitope tag, N-DYKDDDDK-C, is the first epitope tag designed for fusion proteins and is the only patented tag. The multiple polyanionic amino acids in the FLAG tag are less likely to affect the activity of a target protein….Figure 1.
| tag | num |
|---|---|
| FLAG | 236 |
| Myc | 218 |
| GFP | 166* |
| HA | 164 |
What is FLAG peptide used for?
FLAG peptide is an eight amino acids peptide (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) with an enterokinase-cleavage site; designed for antibody-mediated identification and purification of recombinant proteins.
How big is an HA tag?
The hemagglutinin (HA) tag is a 9-amino acid long peptide corresponding to residues 98-106 of the human influenza HA molecule, an ~63kDa surface glycoprotein required for the infectivity of the human virus.
What is HA peptide used for?
HA Peptide (HA tag) is a nine amino acids peptide derived from the human influenza hemagglutinin (HA). HA Peptide is extensively used to isolate, purify, detect, and track the protein of interest in cell biology and biochemistry.
Can peptides be used with hyaluronic acid?
Peptides work well in tandem with other ingredients, including vitamin C, niacinamide (but don’t use niacinamide and vitamin C together!), antioxidants, and hyaluronic acids. Using a peptide with an alpha hydroxy acid (AHA) will actually make the peptides work less efficiently. Look at the label.