Can RedSafe stain RNA?
RedSafe™ Nucleic Acid Staining Solution (20,000x) is a new and safe nucleic acid staining solution. It is an alternative to the traditional ethidium bromide(EtBr) stain for detecting nucleic acid in agarose gels. It emits green fluorescence when bound to DNA or RNA.
Does SYBR Safe work with RNA?
SYBR Safe stain is specifically formulated to be a less hazardous alternative to ethidium bromide that can be used with either blue-light or UV excitation. The stain is also suitable for staining RNA in gels.
What is the function of GelRed?
GelRed is a sensitive, stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EtBr) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels.
How does the RedSafe stain allow for the visualization of nucleic acids in an agarose gel?
RedSafeTM Nucleic Acid Staining Solution is a safe nucleic acid stain, an alternative to the traditional ethidium bromide(EtBr) stain for detect ing nucleic acid in agarose gels. It emits green fluorescence when bound to DNA or RNA. The fluorescence emission of RedSafe bound to DNA is centered at 537 nm.
What is the purpose of using Redsafe solution in gel electrophoresis?
Note : RedSafeTM Nucleic Acid Staining Solution (20,000x) allows visualization of DNA(>50 ng) in the agarose gel under visible light. This eliminates the need for exposure to UV light, which may nick and damage DNA.
Does SYBR Green bind RNA?
All SYBR dyes bind to dsDNA, ssDNA and RNA but vary in sensitivity. SYBR Safe DNA Gel Stain was specifically developed as a safer alternative to ethidium bromide. All SYBR dyes bind to dsDNA, ssDNA and RNA but vary in sensitivity. SYBR Green I is used for staining dsDNA and ssDNA.
How do you remove SYBR Green from DNA?
SYBR Safe DNA Gel Stain is easily removed from nucleic acids by ethanol precipitation.
What is GelRed binding?
GelRedTM is a much heavier dye than EtBr, and GelRed’s affinity for DNA means that the more DNA that is loaded in a gel, the more GelRedTM will bind to DNA and skew the movement of that fragment.
Why is Red safe used during electrophoresis?
Safe-Red™ is a new and safe nucleic acid stain for the visualization of nucleic acids in agarose and polyacrylamide gels. This dye eliminates the need for toxic Ethidium Bromide (EtBr, a potent mutagen), commonly used in gel electrophoresis.
How do I use RedSafe?
Prepare a 100 ml of agarose gel solution (concentration from 0.8~3 %) in a 250 ml flask and mix it thoroughly. Place the flask in the microwave, heat in until the solution is completely clear (about 2~3 minutes). Note : The thickness of gel should be less than 0.5 cm since thick gels may decrease sensitivity. 2.
How is redsafetm used for nucleic acid staining?
Detect the bands under UV illumination. Note : RedSafeTM Nucleic Acid Staining Solution (20,000x) allows visualization of DNA (>50 ng) in the agarose gel under visible light. This eliminates the need for exposure to UV light, which may nick and damage DNA.
How is RNA interference used in everyday life?
RNA interference has been used for the studies in bread wheat. With the invention of synthetically made small interfering RNA, it became possible to silence the specific gene sequences instead of silencing the entire gene. Since then, RNAi has been used to target specific gene sequences that can cause cancer.
How does RNA interference ( RNAi ) regulate protein coding genes?
Introduction RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is a conserved biological response to double-stranded RNA that mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
Which is more sensitive redsafe or EtBr staining solution?
The fluorescence emission of RedSafe™ bound to DNA is at 537 nm. RedSafeTM Nucleic Acid Staining Solution (20,000x) is as sensitive as EtBr.