Can C terminal be phosphorylated?
Our results show that phosphorylation of the C-terminal region of both chaperones is critical for the decision between folding or degradation of client proteins by altering the binding of CHIP and HOP. We also show increased phosphorylation in cancer cells together with increased levels of the co-chaperone HOP.
What is the CTD domain?
The carboxy-terminal domain (CTD) of RNA polymerase II (Pol II) is composed of repeats of the heptapeptide Tyr-Ser-Pro-Thr-Ser-Pro-Ser and is dynamically post-translationally modified to regulate transcription. CTD phosphorylation states are associated with and regulate distinct stages of the transcription process.
What does CTD phosphorylation do?
The phosphorylation of the CTD is an important regulation mechanism, as this allows attraction and rejection of factors that have a function in the transcription process. The CTD can be considered as a platform for transcription factors.
What is the C-terminal amino acid?
A peptide has two ends: the end with a free amino group is called the N-terminal amino acid residue. The end with a free carboxyl group is called the C-terminal amino acid residue. Peptides are named from the N-terminal acid residue to the C-terminal amino acid.
What does the CTD do in transcription?
The carboxy-terminal domain (CTD) of RNA polymerase II is that portion of the polymerase that is involved in the initiation of DNA transcription, the capping of the RNA transcript, and attachment to the spliceosome for RNA splicing.
What does the C-terminal domain do?
RNA polymerase II, and specifically the C-terminal domain (CTD) of its largest subunit, has been demonstrated to play important roles in capping, splicing, and 3′ processing of mRNA precursors.
What is C-terminal analysis?
A chemical method for determination of C-terminal residues of proteins by cleavage by hydrazinolysis, which generates amino acid hydrazides of all amino acids except the C-terminal one; also an enzymic method using limited cleavage by a carboxypeptidase that sequentially liberates amino acid residues from the C- …