Why is lipemic sample not used in serologic testing?
The possible mechanism of interference by hemolysis or lipemia in serologic assays may be as follows: Interference with antigen-antibody reaction. Very severe lipemia may make it difficult for accurate volume measurement in assays requiring precise sample measurement. Interference with color measurement in assays.
What tests are affected by lipemia?
Conclusion: Lipemia causes clinically significant interferences for phosphorus, creatinine, total protein and calcium measurement and those interferences could be effectively removed by ultracentrifugation.
Does Hemolyzed sample affect CRP?
In the present study, hemolysis produced significant changes in CRP and Cp values.
What causes lipemia in blood samples?
The most common cause of lipemia is nonfasting, with recent ingestion of lipid-containing meal. More severe lipemia results from a disease condition causing hypertriglyceridemia (eg, diabetes, genetic hyperlipidemia) or recent intravenous infusion of a lipid emulsion.
What is a lipemic specimen?
Lipemic samples are patient specimens that have a higher fat content in them, so their blood is a little more milky, thicker. Whereas, usually when you spin down whole blood, it will look like this. It has yellow, clear serum or plasma on top of the red cells. This is plasma here on top of the red blood cells.
Is lipemic serum sample accepted for triglyceride measurement?
Lipemia can be detected visually if the concentration of triglycerides in patient sample is over 3.4 mmol/L (32). Lipemia of these samples was not detected visually when samples were analyzed, but only by measuring L Index. Visual detection of lipemia in the serum gives highly heterogeneous results.
What is a lipemic sample?
Lipemic samples are patient specimens that have a higher fat content in them, so their blood is a little more milky, thicker. Whereas, usually when you spin down whole blood, it will look like this. It has yellow, clear serum or plasma on top of the red cells.
What chemistries are affected by hemolysis?
Potassium, aspartate transaminase (AST), amylase, calcium, phosphorus, magnesium, total protein, and total and direct bilirubin showed significant changes caused by hemolysis.
Does Hemolyzed sample affect creatinine?
Specimen and Processing Considerations On the other side, hemolysis may falsely decrease creatinine, alkaline phosphatase (ALP), potassium, packed cell volume (PCV), and mean corpuscular volume (MCV).
What does lipemic blood sample mean?
Lipemia is presence of a high concentration of lipids (or fats) in the blood. When donated blood is lipemic it causes the plasma-containing products to have a milky appearance.
What is a lipemic blood sample?
What Are Lipemic Samples. Lipemic samples are patient specimens that have a higher fat content in them, so their blood is a little more milky, thicker. Whereas, usually when you spin down whole blood, it will look like this. It has yellow, clear serum or plasma on top of the red cells.
What is meant by lipemic?
Lipemia is defined as visible turbidity in serum or plasma samples due to the presence of lipoprotein particles, especially chylomicrons. The most common cause of turbidity is a high concentration of triglycerides [1,2].