What is the size of globular proteins?
Table 4
| Protein | Dimensions (nm) | S max |
|---|---|---|
| Globular protein standards dimensions are from pdb files | ||
| Phosphofructokinase | 14 × 9 × 9 | 17.77 |
| Catalase | 9.7 × 9.2 × 6.7 | 13.6 |
| Serum albumin | 7.5 × 6.5 × 4.0 | 5.9 |
What does SEC MALS measure?
SEC-MALS determines molecular weight from 200 g/mol to 1 billion g/mol. It can also determine molecular size – the rms radius, or radius of gyration Rg – from 10 nm to 500 nm and beyond. By combining molar mass and size, it can also assess molecular conformation.
What is MALS used for?
Multiangle light scattering (MALS) describes a technique for measuring the light scattered by a sample into a plurality of angles. It is used for determining both the absolute molar mass and the average size of molecules in solution, by detecting how they scatter light.
How do you determine the size of a protein?
Whatever program you use to visualize protein structures should have tool to measure the distance between any 2 atoms in the structure. Find the 2 atoms that appear to be the farthest apart, and measure the distance between them. That is the size of the protein in that dimension.
What is the average size of a protein?
Eukaryotic proteins have an average size of 472 aa, whereas bacterial (320 aa) and archaeal (283 aa) proteins are significantly smaller (33-40% on average).
How does RI detector work?
The detector monitors refractive index either by measuring the deflection of a light beam passing through an interface with the eluent or by measuring the amount of light reflected by the interface at angles near the critical angle.
What is MALS detector?
A multi angle light scattering (MALS) detector is a form of static light scattering detector which allows the absolute molecular weight (Mw) and potentially the radius of gyration (Rg) of a sample to be measured.
How small is a protein molecule?
A simple rule of thumb for thinking about typical soluble proteins like the Rubisco monomer is that they are 3-6 nm in diameter as illustrated in Figure 1 which shows not only Rubisco, but many other important proteins that make cells work.