What is R26 mice?
The R26 locus was first identified in a gene trapping experiment in mouse embryonic stem cells [13]. We expect this dual fluorescent reporter mouse will be a useful tool in developmental biology studies, stem cell and cancer initiating cell lineage tracing as well as transplantation experiments.
What is a Cre-reporter mice?
Abstract. The Cre/loxP system is a strategy for controlling temporal and/or spatial gene expression through genome alteration in mice. As successful Cre/loxP genome alteration depends on Cre-driver mice, Cre-reporter mice are essential for validation of Cre gene expression in vivo.
How Cre loxP mice work?
The Cre protein is a site-specific DNA recombinase that can catalyse the recombination of DNA between specific sites in a DNA molecule. These sites, known as loxP sequences, contain specific binding sites for Cre that surround a directional core sequence where recombination can occur.
What is GFP reporter?
Green fluorescent protein is a quantitative reporter of gene expression in individual eukaryotic cells.
What is loxP site?
LoxP sites are directional 34 bp sequences made up of two 13 bp recognition sites separated by an 8 bp spacer region. The sequences don’t occur naturally in any known genomes other than the P1 bacteriophage, and are long enough that they are unlikely to occur by chance.
How is Cre mouse created?
Cre-loxP system is a widely used powerful technology for mammalian gene editing. Conditional knockout mice are then generated by breeding the Cre-driver strain with a floxed mouse strain (Figure 1B). The specificity and timing of recombination are controlled by used promoter and/or enhancer.
How do I check my GFP fluorescence?
Flow cytometry and fluorescent microscopy are two conventional tools to detect the GFP signal; flow cytometry is an effective and sensitive technique to quantitatively analyze fluorescent intensity, while fluorescent microscopy can visualize the subcellular location and expression of GFP.
What happens to r26-stop-eYFP mutant mice?
Mice that are homozygous for the R26-stop-EYFP mutant allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These mice contain an Enhanced Yellow Fluorescent Protein gene (EYFP) inserted into the Gt(ROSA)26Sor locus. Expression of EYFP is blocked by an upstream loxP-flanked STOP sequence.
How are ROSA26 knock-in mice used in research?
In the early 1990s, researchers isolated Rosa26, giving scientists a specific site for inserting genes to study. Prior to this, geneticists used transgenic mouse models to test hypotheses. Transgenic mice are generated by injecting plasmid DNA into a pronucleus.
Is the ROSA26 locus found in humans or mice?
Although the Rosa26 locus was originally characterized in mice, it is also present in humans, pigs, rats, mice and rabbits. In 2018, researchers successfully identified the Rosa26 site in bovines.
How long does it take to recover a r26r mouse?
When using the R26R mouse strain in a publication, please cite the originating article (s) and include JAX stock #009427 in your Materials and Methods section. Typically mice are recovered in 10-14 weeks. Contact Customer Service to place an order or for more information.