What is MaxiSorp plate?
The MaxiSorp surface is a modified, highly charged polystyrene surface with high affinity to molecules with polar or hydrophilic groups. The surface has a high binding capacity for proteins, including globular antibodies in proper orientation. For the PolySorp surface, the binding capacity is 220 ng/cm2.
What are Nunc plates?
Thermo Scientific™ Nunc MicroWell 96-Well Microplates are optimized for robotics, automated readers and liquid handling systems. 96-well microplates are used for antibiotic screens, cell-based assays and screening compounds. The flat bottom shape is ideal for microscopic and optical measurements.
Why are MaxiSorp plates used for an Elisa?
The MaxiSorp surface is a hydrophilic/hydrophobic mix that binds to a wide variety of biomolecules, making it a great choice to bind most anitgens or primary antibodies during ELISA.
What is the purpose of a well plate?
noun. A rectangular plate or block containing multiple small wells or cavities, used for analysis, clinical testing, or culture; specifically = microtiter plate .
How do MaxiSorp plates work?
The MaxiSorp surface is a modified, highly charged polystyrene surface with high affinity to molecules with polar or hydrophilic groups. The surface has a high binding capacity for proteins, including globular antibodies in proper orientation. Thus, it offers very high sensitivity in double antibody “sandwich” tests.
What is Elisa protocol?
An enzyme-linked immunosorbent assay (ELISA) is used to detect the presence of an antigen in a sample. The antigen is immobilized to the well of a plate by adsorption, or captured with a bound, antigen-specific antibody. A detection antibody is then added forming a complex with the antigen, if present.
How much media is in a 10cm plate?
Add fresh medium, resuspend the cells, and then dilute in a larger flask to an appropriate volume (25 mL per 15 cm plate, 10 mL per 10 cm plate) with growth medium and plate on new dishes.
How much media do you put in a 96-well plate?
Useful information for various sizes of cell culture dishes and flasks
| Catalog No. | Growth medium (mL). Approx. volume | |
|---|---|---|
| Dishes | ||
| 24-well | 142475 | 0.5 to 1.0 |
| 48-well | 150687 | 0.2 to 0.4 |
| 96-well | 167008 | 0.1 to 0.2 |
How much can 48 well plate hold?
Useful information for various sizes of cell culture dishes and flasks
| Catalog No. | Versene (mL of 0.05% EDTA). Approx. volume | |
|---|---|---|
| Dishes | ||
| 12-well | 150628 | 0.4 to 1 |
| 24-well | 142475 | 0.2 to 0.3 |
| 48-well | 150687 | 0.1 to 0.2 |
What are microplates used for?
The microplate is designed to measure absorbance in small volumes. It is typically used to determine the concentration of nucleic acids and proteins. It contains 16 spots which can be loaded with 2 microliters of different samples or replicates for subsequent absorbance measurements.