What is anti myc antibody?
The Myc tag, derived from the human c-myc gene, is a peptide epitope tag used in detection or purification of recombinant proteins. Antibodies to the myc tag can be used in the analysis of myc-tagged proteins. …
How does a myc tag work?
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism.
How many myc tags are there?
The Myc-tag can be used for many capture and detection applications such as immunoprecipitation, immunofluorescence and protein purification. 1. What is Myc- tag?…12. Myc-tag vs. Spot-Tag®
| Myc-tag | Spot-Tag | |
|---|---|---|
| Origin | human c-Myc | Derived from human beta-catenin |
| 3D Structure | ||
| Amino acid sequence | EQKLISEEDL | PDRVRAVSHWSS |
What is DDK tag?
DYKDDDDK Tag (FLAG® tag, FLAG octapeptide) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. If there is no antibody against the studied protein, adding a FLAG tag to this protein allows one to follow the protein with an antibody against the FLAG sequence.
What is myc tag used for?
The Myc tag, derived from the c-Myc protein, is a popular epitope tag for detecting the expression of recombinant proteins in yeast, bacteria, insect, and mammalian cell systems. The Myc tag may be fused to either the N-terminus or C-terminus of a protein.
What is a V5 tag?
The V5 tag is derived from a small epitope (Pk) found on the P and V proteins of the paramyxovirus of the simian virus 5 (SV5) family. V5 tag antibodies provide a dependable method for the detection and purification of tagged target proteins without a protein-specific antibody or probe.
Why is Myc used as a tag?
Is C-MYC the same as Myc?
The Myc family consists of three related human genes: c-myc (MYC), l-myc (MYCL), and n-myc (MYCN). c-myc (also sometimes referred to as MYC) was the first gene to be discovered in this family, due to homology with the viral gene v-myc.
Why is myc used as a tag?
Is C-MYC the same as myc?
Is c-myc the same as Myc?
Where is the V5 antigen located?
The V5 tag is derived from a small epitope (Pk) found on the P and V proteins of the paramyxovirus of the simian virus 5 (SV5) family. The V5 tag is generally used with all 14 amino acids (GKPIPNPLLGLDST), but it may also be used with a shorter 9-amino acid (IPNPLLGLD) sequence.